| AVS 59th Annual International Symposium and Exhibition | |
| Applied Surface Science | Wednesday Sessions |
| Session AS-WeM |
| Session: | Surface Analysis of Biological Materials Using Vibrational & Non Linear Optical Spectroscopy Techniques (8:00-10:00 am) / 3D Imaging & Nanochemical Analysis - Part 1 (10:40 am-12:00 pm) |
| Presenter: | M. Unger, University of Wisconsin Milwaukee |
| Authors: | M. Unger, University of Wisconsin Milwaukee E. Mattson, University of Wisconsin Milwaukee J. Sedlmaier, University of Wisconsin Milwaukee Z. Alavi, University of Wisconsin Milwaukee R. Dsouza, University of Wisconsin Milwaukee B. Manandar, University of Wisconsin Milwaukee C. Hirschmugl, University of Wisconsin Milwaukee |
| Correspondent: | Click to Email |
FT-IR spectrochemical imaging, which combines the chemical specificity of mid-infrared spectroscopy with spatial specificity, is an important demonstration of label-free molecular imaging. Mid-infrared optical frequencies are resonant with the vibrational frequencies of functional groups, thus an absorption spectrum is a “molecular fingerprint” of the material at every pixel. Each spectrum can be correlated with known material properties to extract chemical information. Synchrotron based FT-IR spectrochemical imaging, as recently implemented at the Synchrotron Radiation Center in Stoughton, WI, demonstrates the new capability to achieve diffraction limited chemical imaging across the entire mid-infrared region, simultaneously, with high signal to noise ratio.
IRENI [1] (Infrared Environmental Imaging) extracts a large swath of radiation (320 hor. × 25 vert. mrads2) to homogeneously illuminate a commercial IR microscope equipped with an infrared Focal Plane Array (FPA) detector. Wide field images are collected. IRENI rapidly generates high quality, high spatial resolution data. The relevant advantages (spatial oversampling, speed, sensitivity and signal to noise ratio) will be presented and demonstrated using examples from a variety of disciplines, including formalin fixed [1] and flash frozen tissue samples [2], live cells, fixed cells, paint cross sections and polymer fibers will be presented.
* This work has been done with support from NSF (MRI-DMR-0619759 and CHE-1112433) and the Synchrotron Radiation Center, which is also supported by NSF (DMR-0537588) and UW-Milwaukee and UW-Madison.
[1] M.J. Nasse, et al. “High resolution Fourier-transform infrared chemical imaging with multiple synchrotron beams”, Nature Methods, 8, (2011) 413-416
[2] M.Z. Kastyak-Ibrahim, et al. “Biochemical label-free tissue imaging with subcellular –resolution synchrotron FTIR with Focal Plane Array Detector,” NeuroImage 60, (2012) 376-383.