AVS 53rd International Symposium
    Nucleic Acids at Surfaces Topical Conference Monday Sessions
       Session DN-MoM

Paper DN-MoM4
Amino-Silane and Chitosan Coated Silicon Substrates for DNA Extraction

Monday, November 13, 2006, 9:00 am, Room 2014

Session: Nucleic Acids at Surfaces I
Presenter: A.M. Anderle, ITC-irst, Italy
Authors: P.L. Pasquardini, ITC-irst, Italy
F.S. Fiorilli, Politecnico di Torino, Italy
F.S. Forti, ITC-irst, Italy
L. Lunelli, ITC-irst, Italy
P.C. Pederzolli, ITC-irst, Italy
V.L. Vanzetti, ITC-irst, Italy
V.M. Vinante, ITC-irst, Italy
G.E. Garrone, Politecnico di Torino, Italy
A.M. Anderle, ITC-irst, Italy
Correspondent: Click to Email
Lab-on-a-chip technology has been recently attracting much attention due to their increasing applications to biomedical and clinical diagnosis based on genomic analysis. To this purpose, different methods to perform DNA extraction on a chip have been proposed. Recently, amino-coated silicon microchip has been found to show high capacity to bind DNA, due to the occurrence of electrostatic interaction between amine groups and nucleic acids.@footnote 1@ In this contribution a study about the binding efficiency of DNA on amino-modified silicon and about the quantification of recovered DNA is reported. Amine groups were introduced on silicon substrate by coating with an amino-silane molecule and with chitosan. Silicon substrates have been initially covered with silicon oxide grown both thermally and by PECVD. Aminopropyltriethoxysilane (APTES) has been used as organosilane agent to introduce amine groups. Alternatively a natural polymer, chitosan, carrying amines with a lower pK (~6.3)@footnote 2@ was deposited by evaporation from acid solutions. Amino-coated substrates have been characterized by contact angle, AFM, ESCA and fluorescence microscopy. Genomic DNA (extracted from whole blood) and ï¬-phage DNA molecules adhesion was monitored on the surfaces by AFM and fluorescence microscopy and their release in different elution conditions was quantified by fluorescence spectroscopy. @FootnoteText@@footnote 1@T.Nakagawa, T. Tanaka, D. Niwa, T. Osaka, H. Takeyama, T. Matsunaga, Journal of Biotechnology 116 (2005) 105-111 @footnote 2@L. Wu, A. P. Gadre, H. Yi, M. J. Kastantin, G. W. Rubloff, W. E. Bentley, G. F. Payne, and R. Ghodssi, Langmuir 18 (2002) 8620-8625.