| AVS 65th International Symposium & Exhibition | |
| Applied Surface Science Division | Tuesday Sessions |
| Session AS+BI-TuM |
| Session: | Applied Surface Science: From Electrochemistry to Cell Imaging, a Celebration of the Career of Nicholas Winograd |
| Presenter: | John Stephen Fletcher, University of Gothenburg, Sweden |
| Authors: | J.S. Fletcher, University of Gothenburg, Sweden I. Kaya, University of Gothenburg, Sweden |
| Correspondent: | Click to Email |
Despite imaging SIMS being a much older technique than MALDI in the bio-MS imaging area MALDI has enjoyed considerably more widespread success. The advantage of higher resolution imaging that is possible with SIMS has generally been outweighed by the low signal for intact molecular ions that are routinely delivered by soft ionisation techniques like MALDI – and MALDI is cheaper. However, advances in ion beams and mass spectrometry for SIMS analysis in parallel with new matrices, sample preparation and analysis approaches for MALDI have brought the two techniques closer together with significant overlap in the 1-10 µm “small molecule” imaging range.
In this presentation the benefits (if any) of multimodal MS imaging are discussed using examples from cancer, cardiovascular and neurological studies. Analysis was performed using high energy (40 keV) gas cluster ion beams (GCIBs) for SIMS analysis on the Ionoptika J105 and different MALDI approaches including gentle/static MALDI on the Bruker Ultraflextreme. On tissue derivatisation strategies applicable to both techniques will also be presented.