| AVS 61st International Symposium & Exhibition | |
| Spectroscopic Ellipsometry Focus Topic | Friday Sessions |
| Session EL+AS+BI+EM+SS-FrM |
| Session: | Application of SE for the Characterization of Organic and Biological Materials |
| Presenter: | DaeWon Moon, DGIST, Republic of Korea |
| Correspondent: | Click to Email |
Understanding interfacial phenomena has been one of the main research issues not only in semiconductors but only in life sciences. I have been trying to meet the atomic scale surface and interface analysis challenges from semiconductor industries and furthermore to extend the application scope to biomedical areas. Optical imaing has been most widely and successfully used for biomedical imaging but complementary mass spectrometric imaging can provide more detailed molecular specific information
In this presentation, I report our recent activities of multimodal nanobio imaging of cardiovascular cells and tissues. Firstly, in atherosclerotic plaque imaging using coherent anti-stokes raman scattering (CARS) and time-of-flight secondary ion mass spectrometry (TOF-SIMS), multimodal CARS & SIMS analysis showed that increased cholesterol palmitate may contribute to the formation of a necrotic core by increasing cell death. Secondly, surface plasmon resonance imaging ellipsometry (SPRIE) was developed for cell biointerface imaging of cell adhesion, migration, and infiltration dynamics for HUVEC, CASMC, and T cells. SPRIE images were validated with confocal fluorescence microscopy. Collagen fibrils are widely used as cell adhesion substrates. Changes of surface composition and elastic modulus of collagen fibrils after thermal and acidic treatment were investigated by TOF-SIMS and non-contact force microscopy. Multimodal SPRIE & TOF-SIMS imaging would be a useful methodology for understanding cell-substrate interactions in tissue engineering.
In conclusions, multimodal optical and mass spectrometric imaging privides overall structural and morphological information with complementary molecular specific information, which can be a useful methodology for biomedical studies. Future challenges in optical and mass spectrometric imaging for new biomedical applications will be discussed regarding to in-vivo imaging.