AVS 59th Annual International Symposium and Exhibition
    Scanning Probe Microscopy Focus Topic Wednesday Sessions
       Session SP+AS+BI+ET+MI+NM+NS+SS+TF-WeM

Invited Paper SP+AS+BI+ET+MI+NM+NS+SS+TF-WeM11
Nanoscale Surface Assembly by Single-Molecule Cut-and-Paste

Wednesday, October 31, 2012, 11:20 am, Room 16

Session: Probe-Sample Interactions, Nano-Manipulation and Fabrication
Presenter: H.E. Gaub, Ludwig-Maximilians Universitat, Germany
Correspondent: Click to Email
Bottom up assembly of functional molecular ensembles with novel properties emerging from composition and arrangement of its constituents is a prime goal of nanotechnology. With the development of Single-Molecule Cut-and-Paste (SMC&P) we provided a platform technology for the assembly of biomolecules at surfaces. It combines the Å-positioning precision of the AFM with the selectivity of DNA hybridization to pick individual molecules from a depot chip and allows to arrange them on a construction site one by one. An overview on different applications of this technology will be given in this talk. One recent example demonstrates the functional of receptors for small molecules. By SMC&P we assembled binding sites for malachite green in a molecule-by-molecule assembly process from the two halves of a split aptamer. We show that only a perfectly joined binding site immobilizes the fluorophore and enhances the fluorescence quantum yield by several orders of magnitude. To corroborate the robustness of this approach we produced a micron-sized structure consisting of more than 500 reconstituted binding sites. To the best of our knowledge this is the first demonstration of a one by one bottom up functional bio-molecular assembly. Figure included in supplemental document. S. Kufer, Puchner E. M.,Gumpp H., Liedel T. & H. E. Gaub Science (2008), Vol 319, p 594-S. Kufer, Strackharn, M., Stahl S.W., Gumpp H., Puchner E. M. & H. E. Gaub Nature Nanotechnology (2009), Vol 4, p 45-M. Erdmann, R. David. A.N. Fornof, and H. E. Gaub, Nature Chemistry (2010), Vol 2, p 755-M. Strackharn, S. Stahl, E. Puchner & H.E. Gaub, Nanoletters (2012) in press