AVS 53rd International Symposium
    Applied Surface Science Monday Sessions
       Session AS+BI+NS+NM-MoM

Paper AS+BI+NS+NM-MoM10
Tailoring of Functional Nano-Patterned Surfaces for Biosensing Applications by Combination of Plasma Processes and Electron-Beam Lithography

Monday, November 13, 2006, 11:00 am, Room 2005

Session: Organic Surface Modification and Nanoscale Chemical Patterning
Presenter: F. Rossi, JRC-IHCP-BMS, Italy
Authors: F. Brétagnol, JRC-IHCP-BMS, Italy
L. Ceriotti, JRC-IHCP-BMS, Italy
A. Valsesia, JRC-IHCP-BMS, Italy
T. Sasaki, JRC-IHCP-BMS, Italy
D. Gilliland, JRC-IHCP-BMS, Italy
G. Ceccone, JRC-IHCP-BMS, Italy
P. Colpo, JRC-IHCP-BMS, Italy
F. Rossi, JRC-IHCP-BMS, Italy
Correspondent: Click to Email
Fabrication of micro and nano-patterned surfaces with a well defined geometry and a controlled chemistry is a fundamental step for the development of bioengineered materials Micro-arranged surfaces containing functionalities such as cell or protein adhesive in a non non-adhesive matrix provide a very useful tool in a large field of applications (tissue engineering, cell behavior investigations , artificial growth of neurons networksâ?¦) . Numerous methods have been successfully developed for the production of such surfaces including conventional photolithography, photochemistry , micro-contact printing , micro fluidic patterning . Nevertheless, the development of surfaces chemically patterned at nano scale is still a challenging issue for the implementation of new generation of miniaturized biochips and for the study of cell surface interactions. In this study, we present an innovative method for the fabrication of chemically nano-patterned surfaces. Maskless lithography based on electron-beam technique was successfully combined with plasma-based processes to create COOH terminated spots over a PEO-like matrix. Spots from micron to submicron size have been created. Quality control of the patterned surface was studied by Atomic Force Microscopy, XPS and ToF-SIMS analysis. Experiments with fluorescent proteins on the patterned surfaces exhibit a preferential adhesion on the active region showing the ability of this technique for the design of biosensing platforms.