AVS 52nd International Symposium
    MEMS and NEMS Monday Sessions
       Session MN-MoP

Paper MN-MoP3
DNA Detection System using a Microcantilever

Monday, October 31, 2005, 5:00 pm, Room Exhibit Hall C&D

Session: General Aspects of MEMS and NEMS Poster Session
Presenter: K.-A. Yoo, Myongji University, Korea
Authors: K.-A. Yoo, Myongji University, Korea
K.-H. Na, Lite-on Technology Corp. Korea
S.-R. Joung, Myongji University, Korea
C.J. Kang, Myongji University, Korea
Y.S. Kim, Myongji University, Korea
Correspondent: Click to Email
We propose a novel detection system for analysis various biotinylated DNAs effectively with a microcantilever. The microcantilevers were fabricated employing surface micromachining technique that has attractive advantages in terms of cost efficiency, simplicity and ability of fabricating in array. The fluid cell system for injection of bio-molecular solution is fabricated using a polydimethylsiloxane (PDMS) and a fused silica glass. The microcantilever is deflected with respect to the difference of the surface stress caused by the formation of self-assembled bio-molecules on the gold coated side of the microcantilever. It can detect various biotinylated DNAs according to the specific interactions between the streptavidin and individual DNA sequencing of biotinylated DNA. We confirm that the deflections of bending-up or bending-down of individual microcantilevers are occurred by the bio-molecule adsorption. The microcantilever detected protein A and DNA due to the specific interaction between protein A and DNA. The principle of the interaction is a self-assembly between the bio-molecules. The microcantilever can be widely used to detect various bio-molecules including specific DNA and can be utilized as a bio-sensor.