AVS 52nd International Symposium
    Biomaterial Interfaces Wednesday Sessions
       Session BI1-WeM

Paper BI1-WeM5
In Deuterated Water the Unspecific Adsorption of Proteins Is Significantly Slowed Down: Results of an SPR-study using Model Organic Surfaces

Wednesday, November 2, 2005, 9:40 am, Room 311

Session: Protein-Surface Interactions
Presenter: Chr. Grunwald, Ruhr-University, Germany
Authors: Chr. Grunwald, Ruhr-University, Germany
J. Kuhlmann, Max-Planck-Institute for Molecular Physiology, Germany
Ch. Woell, Ruhr-University, Germany
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The control of unspecific adsorption of proteins to natural and technical surfaces plays an important role in biology and also for many applications. Organic model surfaces e.g. self-assembled monolayers, are often used to identify fundamental surface and/or protein properties that rule protein adsorption.@footnote 1@ Some techniques involved in biointerface research require the use of heavy water, e.g. neutron scattering techniques.@footnote 2@ Also in NMR studies D@sub 2@O is the solvent of choice when focusing on biomolecular and hydration dynamics. So far several studies have been concerned with the characterization of the unspecific adsorption of proteins from normal water buffers.@footnote 3@ In the present work we report a comparison of the unspecific protein adsorption from normal and heavy water buffers. Previously it has been assumed that the surface kinetic of the unspecific adsorption is unaffected by the substitution of water by D@sub 2@O.@footnote 2@ However, for the four proteins investigated here this assumption does not hold. The ratio k@sub H@/k@sub D@ of the adsorption rate constants of the different buffer conditions describe the strength of the isotope effect. We have measured ratios between 1.0 and 2.6 indicating that the adsorption kinetics are strongly affected by a H@sub 2@O-D@sub 2@O-substitution. @FootnoteText@ @footnote 1@Herrwerth, S.; Eck, W.; Reinhardt, S.; Grunze, M. Journal of the American Chemical Society 2003, 125, 9359-9366.@footnote 2@Schwendel, D.; Hayashi, T.; Dahint, R.; Pertsin, A.; Grunze, M.; Steitz, R.; Schreiber, F. Langmuir 2003, 19, 2284-2293.@footnote 3@Ostuni, E.; Grzybowski, B. A.; Mrksich, M.; Roberts, C. S.; Whitesides, G. M. Langmuir 2003, 19, 1861-1872.