Paper AS-MoP3
Synthesis and Characterization of Spectroscopic Tags Based Surface-Enhanced Raman Spectroscopy (SERS) of BPE Adsorbed on Gold Nanoparticles
Monday, October 31, 2005, 5:00 pm, Room Exhibit Hall C&D
Nanoparticle tags detectable by Raman spectroscopy, due to the surface-enhanced Raman effect (SERS) of adsorbed reporter molecules, have potential applications ranging from biological assays to cellular imaging. This paper describes the surface chemistry considerations, and characterization methods employed, in producing gold nanoparticles with an adsorbed bis(4-pyridyl) ethylene [BPE] reporter molecule. The SERS response of the tags is affected by the size of the gold nanoparticles, the adsorption conditions of the BPE, and co-adsorption of precursors for growth of protective shells. A primary consideration in producing tags is preventing aggregation of the particles, even though the SERS enhancement of individual particles is lower than aggregates. We will present data from Raman spectroscopy, UV-visible absorbance, light scattering, and TEM to used to characterize the tags during the synthesis process.