This presentation will focus on the characterization, derivatization, and some applications of segmented metallic nanowires having stripes of different metals along their length. These particles are prepared by sequential electrodeposition of metals (e.g. Au, Ag, Pd, Pt, Co) within the pores of alumina or polycarbonate templates, and typically range from 30-300 microns in diameter and up to 10 microns in length. They can be released from the template and derivatized with proteins or nucleic acids for use in sensing or assembly. The metal segments can be used as an optical barcode to enable multiplexed bioanalysis or as a chemical pattern to spatially control (bio)chemical derivatization along the length of the particle. Both multiplexed bioanalytical applications and DNA-directed nanowire assembly will be discussed.