AVS 49th International Symposium
    Biomaterials Thursday Sessions
       Session BI+HS+SS-ThM

Paper BI+HS+SS-ThM7
DIOS-MS for Reaction Monitoring and Chemical Analysis

Thursday, November 7, 2002, 10:20 am, Room C-201

Session: Biosensors and Biodiagnostics
Presenter: J.E. Crowell, University of California, San Diego
Authors: Z. Shen, University of California, San Diego
G. Siuzdak, The Scripps Research Institute
M.G. Finn, The Scripps Research Institute
J.E. Crowell, University of California, San Diego
Correspondent: Click to Email
Desorption/Ionization On Silicon Mass Spectrometry (DIOS-MS) is a new mass spectrometry strategy based on pulsed laser desorption/ionization from a porous silicon surface. DIOS-MS is similar to matrix-assisted laser-desorption ionization mass spectrometry (MALDI-MS) in that it utilizes the same instrument; however, in DIOS-MS, porous silicon is used to trap analytes deposited on the surface and laser radiation is used to vaporize and ionize these molecules, without the presence of any matrix material. We have shown that DIOS-MS can be used for a wide range of small molecules as well as biomolecules at the femtomole and attomole level with little or no fragmentation. DIOS-MS offers many unique advantages including good sensitivity, low background ion interference, and high salt tolerance. We will demonstrate the application of DIOS-MS to small molecule quantitative analysis, high throughput screening, chemical reaction monitoring, enzyme-substrate reaction and inhibition characterization, drug metabolism studies, and protein identification. We will also discuss aspects of the desorption and ionization mechanisms of DIOS.