AVS 49th International Symposium
    Biomaterials Thursday Sessions
       Session BI+HS+SS-ThM

Paper BI+HS+SS-ThM11
Engineered Biointerfaces for Protein Biochip Applications

Thursday, November 7, 2002, 11:40 am, Room C-201

Session: Biosensors and Biodiagnostics
Presenter: H.B. Lu, Zyomyx, Inc.
Authors: H.B. Lu, Zyomyx, Inc.
M. Mariano, Zyomyx, Inc.
S. Schweizer, Zyomyx, Inc.
H.M. Tran, Zyomyx, Inc.
L.A. Ruiz-Taylor, Zyomyx, Inc.
H. Hong, Zyomyx, Inc.
H.H.J. Persson, Zyomyx, Inc.
R.L. Cicero, Zyomyx, Inc.
P. Kernen, Zyomyx, Inc.
P. Wagner, Zyomyx, Inc.
Correspondent: Click to Email
Protein biochip technology promises breakthroughs in large-scale protein analysis. Measuring and analyzing protein activities in a highly efficient, miniaturized and parallel fashion requires advanced surface chemistries for reproducible protein immobilization and minimized non-specific adsorption. Controlling the solid-liquid interface of a miniaturized biochip becomes a key step for maintaining protein activity and integrating highly sensitive detection techniques. We present several reactive surfaces engineered for protein biochip applications at Zyomyx. Systematic efforts on designing organic layers on different substrates have been carried out to improve packing density, orientation, and functionality of immobilized capture reagents, as well as to minimize non-specific biomolecule adsorption in complex biological samples. The latter is particularly important for improving detection limits and obtaining meaningful results in multiplex protein assays. To reduce non-specific adsorption and optimize chip performance, we incorporated oligo- and poly-ethylene glycol (EG) molecules in our organic layers that are well known to reduce non-specific protein adsorption. Effects of substrate type, surface coverage, and molecular structure of the assembled organic layers on specific and non-specific interaction of biomolecules with the surfaces are presented. Specificity, loading capacity and detection sensitivity of protein immunoassays using high-density protein arrays configured with these surfaces are demonstrated and discussed.