IUVSTA 15th International Vacuum Congress (IVC-15), AVS 48th International Symposium (AVS-48), 11th International Conference on Solid Surfaces (ICSS-11)
    Plasma Science Monday Sessions
       Session PS2-MoA

Paper PS2-MoA3
Control of Spatial Distribution of Adsorbed Proteins Using Plasma Surfaces

Monday, October 29, 2001, 2:40 pm, Room 104

Session: Plasma Modification of Organics
Presenter: N.A. Bullett, University of Sheffield, U.K.
Authors: N.A. Bullett, University of Sheffield, U.K.
R.D. Short, University of Sheffield, U.K.
C.W.I. Douglas, University of Sheffield, U.K.
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The adsorption of proteins occurs whenever a material contacts with biological media. In cell culture, proteins rapidly adsorb to the plastic from serum and the adsorbed protein layer subsequently influences cell adhesion, proliferation and differentiation. The ability to control and direct specific protein adsorption and conformation would enable culture surfaces to actively influence the behaviour of cells. This work aims to show that we can exert a degree over control of the adsorption and conformation of proteins from both single solution and serum. The ability to control the spatial distribution of adsorbed proteins will also be demonstrated. Plasma-co-polymerisation of acrylic acid with 1,7-octadiene was used to create a range of carboxylic acid functionalised surfaces. Protein adsorption to these surfaces was measured using an antibody recognition technique (ELISA) and radio-labelling, FITC-labelling and XPS analysis of adsorbed proteins. For single protein solutions and serum, radio-labelling, FITC-labelling and XPS showed a decrease in the amount of adsorbed protein binding with surface acid content. ELISA results would appear to show the opposite, more protein binding to the acid functionalised surfaces. This apparent contradiction in data may be explained by a change in the nature of the adsorbed proteins. Chemical micropatterns were created by the plasma polymerisation of either acrylic acid or allyl amine onto 1,7-octadiene through TEM grid masks. Imaging ToF-SIMS and XPS was used to verify the chemical differentiation in the surface. The micropatterned surface was shown to influence the adsorption and spatial distribution of FITC-labelled adsorbed proteins.