AVS 47th International Symposium
    Biomaterial Interfaces Wednesday Sessions
       Session BI-WeP

Paper BI-WeP14
Correlation of Cell Health with Protein Layer Thickness on Modified Surfaces Characterized by XPS, XAS, and Morphological Analysis

Wednesday, October 4, 2000, 11:00 am, Room Exhibit Hall C & D

Session: Poster Session
Presenter: H.E. Canavan, The George Washington University
Authors: H.E. Canavan, The George Washington University
W.E. O'Grady, Naval Research Laboratory
J.J. Hickman, The George Washington University
D.E. Ramaker, The George Washington University
Correspondent: Click to Email
The interactions of biomolecules with surfaces are of significant interest in the areas of biocorrosion, bio-implant rejection, and biological fluid interactions with MEMS devices. This interaction may dictate the health of nearby cells by either a) continuing cell function as normal, b) continuing cell function in an abnormal manner, or c) cell death. In the work presented here, the biomolecular interaction is altered via prior surface modification with Self-Assembled Monolayers (SAMs) of amines or fluorinated compounds. The surfaces are then introduced into cell culture using cardiac and other cells. The interaction of the derivatized surfaces with proteins and cells are investigated to see a differential response to the modifications. X-ray Photoelectron Spectroscopy (XPS) is used as an analytical technique to characterize the modified surfaces of the silanes prior to cell culture. XPS is also used characterize the protein deposition layer thickness which serves as an indication of cell function and health. Optical microscope images of cells grown on different substrates are compared to time-dependent XPS results to correlate cell morphology with protein layer thickness. Sulfur K-Edge X-Ray Absorption Spectroscopy (XAS) data are used to monitor the extent of S-C, S-O, and S-H bonds, which affect the character of the extruded proteins.