AVS 61st International Symposium & Exhibition
    Applied Surface Science Wednesday Sessions
       Session AS+BI+MC-WeA

Invited Paper AS+BI+MC-WeA9
Peter Sherwood Mid-Career Award Talk: Chemical Analysis of Cells and Tissues with Imaging ToF-SIMS

Wednesday, November 12, 2014, 5:00 pm, Room 316

Session: Practical Surface Analysis I
Presenter: Lara J. Gamble, University of Washington
Authors: L.J. Gamble, University of Washington
B. Bluestein, University of Washington
D. Graham, University of Washington
Correspondent: Click to Email
The ability to image cells and tissues with chemical and molecular specificity could revolutionize our understanding of biological processes. It would increase our understanding of chemical changes in cells and tissues as a function of an applied stress or as a result of disease, and enable tracking the spatial distribution of metabolites and lipids. Chemistry of tumor microenvironments, lipid metabolomics relationship to cancer, delivery of nanoparticles to cells, and tissue repair could be visualized on a cellular and sub-cellular level. The sub-cellular resolution mass spectral imaging capability of ToF-SIMS holds the potential to achieve this possibility. ToF-SIMS analysis of biological samples from 2D images of tissue biopsies to 3D images of nanoparticles in cells will be presented including multivariate analysis of the ToF-SIMS image data. The ToF-SIMS images are also combined with optical images of the same samples (same slices and serial biopsy slices). This combination of images allows researchers to visualize a molecular map that correlates with specific biological features or functions. The potential to combine the ToF-SIMS images with other techniques will also be discussed.