| AVS 55th International Symposium & Exhibition | |
| Applied Surface Science | Tuesday Sessions |
| Session AS-TuP |
| Session: | Aspects of Surface Analysis Poster Session |
| Presenter: | I. Ishizaki, ULVAC-PHI, Inc., Japan |
| Authors: | I. Ishizaki, ULVAC-PHI, Inc., Japan N. Sanada, ULVAC-PHI, Inc., Japan S. Iida, ULVAC-PHI, Inc., Japan M. Suzuki, ULVAC-PHI, Inc., Japan Y. Ohashi, ULVAC-PHI, Inc., Japan G. Hayashi, Osaka University, Japan K. Nakatani, Osaka University, Japan |
| Correspondent: | Click to Email |
Although recent XPS instrumental developments would provide high S/N ratio and much improved resolution for better interpretation of chemical structures,1 relatively little application has been reported for bio materials including DNA. It has been reported that XPS N 1s lines of DNA nucleobases were affected by their chemisorption on Cu, and the peak shift was assigned as the dehydrogenation.2 May et al.3 reported in detail on XPS lines for several kinds of DNA and related materials, however, assignment of the N 1s lines in DNA was not fully elucidated as far as our knowledge. In this study, therefore, we studied nitrogen chemical structure of DNA-related molecules using XPS, cluster TOF-SIMS, and DFT calculations. The DNA-related molecules reported here include adenine (A), guanine (G), thymine (T), cytosine (C), and hypoxanthine (I), and their nucleotides, poly(nucleotides), single and double strained DNA molecules, and a DNA microarray. XPS N 1s lines had little differences between nucleobase and nucleotide for each series. On the other hand, they were sharply different between nucleotides and poly(nucleotides) for A, G, and C, but no obvious differences are found for T and I. Comparative study with G and I bases indicated that primary amine (-NH2) group in the molecule played an important role on the chemical state changes of nitrogen. DFT calculation results of model nucleobase crystals and single-strained DNAs will also be discussed.
1 for example, M. C. Biesinger, et al. Surf. Interface Anal., 36 (2004) 1550.
2 M. Furukawa et al. Surf. Sci., 601 (2007) 5433.
3 C.J. May, et al. Anal. Chem., 76 (2004) 1114.