AVS 54th International Symposium | |
Applied Surface Science | Wednesday Sessions |
Session AS+BI+NS-WeA |
Session: | Fabrication and Characterization of Functional Soft Material Surfaces |
Presenter: | P.R. Norton, University of Western Ontario, Canada |
Authors: | P.R. Norton, University of Western Ontario, Canada N. Patrito, University of Western Ontario, Canada J. McLachlan, University of Western Ontario, Canada J. Chan, University of Western Ontario, Canada S. Faria, University of Western Ontario, Canada S. Tadayyon, University of Western Ontario, Canada |
Correspondent: | Click to Email |
Our group has developed a simple protocol to prepare inexpensive, single-component substrates capable of confining cell attachment and growth. In the presence of an argon plasma, thin metal films are deposited onto poly(dimethylsiloxane) (PDMS). Removal of the metal layer exposes regions of the polymer surface that are enriched in oxygen and promote the adhesion of fibroblast, epithelial and myoblast cells. This method produces bioactive arrays of controlled size (down to scales in the order of μm), shape, pitch and symmetry on which cells can be grown to confluency. The treated material is storable and can be activated just before use; this eliminates stability problems inherent in a number of previously reported PDMS surface treatments, most notably oxygen plasma modification. The patterned arrays offer highly adaptable means to probe cell-cell interactions, cell motility and cell signaling in response to varied spatial or geometric organization and they are being incorporated into microfluidic channels for combined optical and proximal probe studies of live cells. Serendipitously, this surface treatment alters the mechanical properties of PDMS, rendering the modified material sensitive to tensile stresses imposed by cells. Cellular traction forces generate nanoscale ripples in the elastic substrata which extend outward from the cell bodies and which can be imaged by dark-field microscopy and AFM. Detailed analyses of these ripples can potentially provide a direct measure of cellular traction forces and mechanical signaling. In related experiments, we have also developed a novel means of bonding PDMS to a host of materials relevant to microfluidic device fabrication, including glass, Si, SiO2 and polystyrene. To quantify the adhesive strength, closed PDMS-glass and PDMS-PDMS microfluidic devices were fabricated and subjected to tensile and leakage testing. The data indicate a significant improvement in performance over previously reported bonding technologies, resulting in the production of more robust, longer-lasting microfluidic devices and the concomitant possibility of using higher pressures and flow-rates.