| AVS 54th International Symposium | |
| Applied Surface Science | Tuesday Sessions |
| Session AS+BI+NS-TuM |
| Session: | Surface Analysis and Related Methods for Biological Materials |
| Presenter: | D.W. Moon, Korea Research Institute of Standards and Science |
| Authors: | D.W. Moon, Korea Research Institute of Standards and Science T.G. Lee, Korea Research Institute of Standards and Science J.Y. Lee, Korea Research Institute of Standards and Science |
| Correspondent: | Click to Email |
Biochemical imaging of cells and tissues is a basic infra-technology in various bio-medical applications. Instead of conventional labeling methodology for biomolecular imaging with fluorescent dyes, label-free single cell and tissue biochemical imaging methodologies such as a nonlinear optical technique, coherent anti-Stokes Raman scattering (CARS) and an ion beam sputtering analysis technique, Secondary Ion Mass Spectrometry (SIMS) using cluster ion beams were developed. They were used to measure in a complementary manner 2D or 3D biochemical images of various cells and tissues such as Hella cells, adipogenic stem cells, fat liver tissues, cancer tissues, and skin tissues. Preliminary results will be discussed on the following issues. 1) Interactions of fibroblasts with native and denatured collagen thin films were studied with CARS and SIMS. It was extended to study the interactions of fibroblasts with 500 nm nano-fibers and 5 μm micro-fiber made of 40% poly (glycolic acid) (PGA) and 60% collagen. 2) Photoaging effects of skin by UV radiation were studied with SIMS, which showed significant changes in the biochemical imaging of amino acids representing collagen fibers and lipid molecules. 3) It was shown that SIMS imaging of colon cancer tissues has some potential to develop personalized cancer therapy with new drugs. Finally, the present status and future prospects of nano-bio technology based on laser, mass spectrometry, and nanoprobe for biochemical imaging of single cells and tissues at KRISS will be discussed for practical applications in bio, medical, and pharmaceutical researches.