| AVS 66th International Symposium & Exhibition | |
| Biomaterial Interfaces Division | Tuesday Sessions |
| Session BI-TuP |
| Session: | Biomaterial Interfaces Posters/Flash Session |
| Presenter: | Nicholas Dennison, Leibniz Institute of Polymer Research Dresden, Germany |
| Authors: | N.R. Dennison, Leibniz Institute of Polymer Research Dresden, Germany R. Zimmermann, Leibniz Institute of Polymer Research Dresden, Germany M. Nitschke, Leibniz Institute of Polymer Research Dresden, Germany V. Magno, Leibniz Institute of Polymer Research Dresden, Germany U. Freudenberg, Leibniz Institute of Polymer Research Dresden, Germany C. Werner, Leibniz Institute of Polymer Research Dresden, Germany |
| Correspondent: | Click to Email |
Chemokines are a class of signalling molecules that play a crucial role in the wound healing process by recruiting immune cells to the affected tissue. In chronic inflammations, this physiological response is prolonged and exacerbated, ultimately causing the destruction of the inflamed tissue and, consequently, the production of more pro-inflammatory chemokines.
One way of interrupting this vicious cycle of chronic inflammations is to remove chemokines from the tissue. Our group has previously demonstrated that hydrogels based on star-shaped polyethylene glycol (starPEG) and desulfated heparin show a promising sequestration pattern of pro-inflammatory chemokines including interleukin-8 (IL-8) and macrophage inflammatory protein-1a (MCP-1) [Lohmann et al. Sci. Transl. Med.9 (2017)].
Analysis of chemokine-loaded hydrogels with time-of-flight secondary ion mass spectrometry (ToF-SIMS) and principle component analysis (PCA) of the obtained spectra allowed for further characterisation of the scavenging process. The results obtained through this methodology were integrated with the enzyme-linked immunosorbent assay (ELISA) detection technique to further optimise the immune-modulating hydrogel.