| AVS 66th International Symposium & Exhibition | |
| Biomaterial Interfaces Division | Wednesday Sessions |
| Session BI+AS-WeM |
| Session: | Microbes and Fouling at Surfaces |
| Presenter: | Jana Schwarze, Ruhr-University Bochum, Germany |
| Authors: | J. Schwarze, Ruhr-University Bochum, Germany K.A. Nolte, Ruhr-University Bochum, Germany R. Wanka, Ruhr-University Bochum, Germany V. Jakobi, Ruhr-University Bochum, Germany A. Rosenhahn, Ruhr-University Bochum, Germany |
| Correspondent: | Click to Email |
Microfluidic environments with laminar flow are a useful tool to quantify attachment and removal of marine biofilm formers and cells1–3. We present results on the microfluidic attachment of the marine bacterium Cobetia marina (C. marina), formerly named Halomonas marina, as it is frequently found in marine biofilms4,5. To identify a suitable shear stress for the microfluidic attachment assays, the attachment behavior of C. marina was investigated at different shear forces on hydrophobic and hydrophilic surfaces, whereby C. marina tends to adhere best on hydrophobic coatings. Among the optimized assay parameters are the relevance of the growth state of C. marina. The optimized assay parameters will be presented as well as selected examples how coating chemistries like different self-assembling monolayers, amphiphilic alginates6 and different thicknesses of PG coatings7 alter the attachment of the marine bacterium C. marina under dynamic shear conditions.
1. Christophis, C., Grunze, M. & Rosenhahn, A. Quantification of the adhesion strength of fibroblast cells on ethylene glycol terminated self-assembled monolayers by a microfluidic shear force assay. Phys. Chem. Chem. Phys. (2010), 12, 4498-4504. doi:10.1039/b924304f
2. Alles, M. & Rosenhahn, A. Microfluidic detachment assay to probe the adhesion strength of diatoms. Biofouling (2015), 31:5, 469-480. doi:10.1080/08927014.2015.1061655
3. Nolte, K. A., Schwarze, J. & Rosenhahn, A. Microfluidic accumulation assay probes attachment of biofilm forming diatom cells. Biofouling (2017), 33 (7): 531-543. doi:10.1080/08927014.2017.1328058
4. Ista, L. K., Fan, H., Baca, O. & López, G. P. Attachment of bacteria to model solid surfaces’ oligo(ethylene glycol) surfaces inhibit bacterial attachment. FEMS Microbiol. Lett. (1996), 142, 59-63. doi:10.1016/0378-1097(96)00243-1
5. Arpa-Sancet, M. P., Christophis, C. & Rosenhahn, A. Microfluidic assay to quantify the adhesion of marine bacteria. Biointerphases (2012), 7, 26. doi:10.1007/s13758-012-0026-x
6. Jakobi, V. et al. Amphiphilic Alginates for Marine Antifouling Applications. Biomacromolecules (2018), 19, 402-408. doi:10.1021/acs.biomac.7b01498
7. Wanka, R. et al. Fouling-Release Properties of Dendritic Polyglycerols against Marine Diatoms. ACS Appl. Mater. Interfaces (2018), 10, 34965–34973. doi:10.1021/acsami.8b12017