| AVS 66th International Symposium & Exhibition | |
| Biomaterial Interfaces Division | Wednesday Sessions |
| Session BI+AS-WeM |
| Session: | Microbes and Fouling at Surfaces |
| Presenter: | Camilo Jaramillo, University of Illinois at Urbana-Champaign |
| Authors: | C. Jaramillo, University of Illinois at Urbana-Champaign A.F. Civantos, University of Illinois at Urbana-Champaign A. Mesa, University of Illinois at Urbana-Champaign J.P. Allain, University of Illinois at Urbana-Champaign |
| Correspondent: | Click to Email |
Silver nanoparticles (Ag NPs) possess remarkable antibacterial properties that are widely recognized. The emergence of antibiotic-resistant bacteria has motivated the interest of Ag NPs as an alternative for antimicrobial protection, in a wide range of applications [1]. However, Ag NPs have also shown toxicity and low biocompatibility. In addition, their synthesis usually involves toxic compounds, further limiting their applicability as a biomaterial. Research on Ag NPs has largely focused on increasing their biocompatibility. Properties such as NPs size, dispersity, and stability have shown to play an important role on their biocompatibility [2]. Green synthesis methods that require non-toxic agents while giving control over these properties are of high interest.
Chitosan (CS) is a deacetylated derivative of chitin, a widely available polymer. Its properties include biodegradability, biocompatibility and non-toxicity, making it an attractive alternative for biomaterials. CS has been used as a bioactive coating (for proteins, drugs and antibiotics and as a stabilizing agent in the production of Ag NPs [3]. Approaches to synthesize CS-based Ag NPs include γ irradiation and sonochemical methods [4].
In this work, Directed Liquid-Plasma Nanosynthesis (DLPNS) was used to drive Ag NPs synthesis without the need of other reagents. CS membranes were used to immobilize the NPs, to explore their application as an antibacterial coating for biomaterials. The Ag NPs precursor concentration and irradiation time were used as control parameters. Surface topography and chemistry were studied by SEM, Contact Angle, XRD and EDS. Antimicrobial properties of the membranes were evaluated against gram-positive (S. aureus) and gram-negative (E. coli) bacteria. Life and death assays revealed the antibacterial activity of the membranes. To study their biocompatibility and cytotoxicity, mammalian cell cultures were used. Cell viability, adhesion and metabolism were evaluated via Alamar blue and immunostaining tests. SEM images were used to assess the presence of Ag NPs in the CS matrix, and observe the bacteria and cell morphology on the surface of the membranes.
[1] D. Wei, W. Sun, W. Qian, Y. Ye, X. Ma, Carbohydr. Res. 344 (2009) 2375–2382.
[2] E.I. Alarcon, M. Griffith, K.I. Udekwu, eds., Silver Nanoparticle Applications, Springer International Publishing, Cham, 2015.
[3] A. Civantos, E. Martínez-Campos, V. Ramos, C. Elvira, A. Gallardo, A. Abarrategi, ACS Biomater. Sci. Eng. 3 (2017) 1245–1261.
[4] N.M. Huang, S. Radiman, H.N. Lim, P.S. Khiew, W.S. Chiu, K.H. Lee, A. Syahida, R. Hashim, C.H. Chia, Chem. Eng. J. 155 (2009) 499–507.