| AVS 66th International Symposium & Exhibition | |
| Biomaterial Interfaces Division | Tuesday Sessions |
| Session BI+AS-TuM |
| Session: | Characterization of Biological and Biomaterial Surfaces |
| Presenter: | Valentina Magno, Leibniz Institute of Polymer Research Dresden, Germany |
| Authors: | V. Magno, Leibniz Institute of Polymer Research Dresden, Germany M. Nitschke, Leibniz Institute of Polymer Research Dresden, Germany R. Zimmermann, Leibniz Institute of Polymer Research Dresden, Germany N.R. Dennison, Leibniz Institute of Polymer Research Dresden, Germany C. Werner, Leibniz Institute of Polymer Research Dresden, Germany, Deutschland |
| Correspondent: | Click to Email |
Decellularized extracellular matrix (ECM) preparations provide highly valuable options for the in vitro reconstitution of tissue-specific niches. In this approach, control over the ECM composition and structural assembly can be achieved through the modulation of cell culture conditions. We have previously demonstrated that adding ascorbic acid and using macromolecular crowding (MMC) allows for tuning the ECM deposition by human mesenchymal stem cells by boosting procollagen synthesis and enhanced complexation/deposition of soluble matrix components [Prewitz et al. Biomaterials 73 (2015) 60]. Combining both options, we have now explored the fabrication of a large set of cell-derived ECM variants which were analyzed by time-of-flight secondary ion mass spectrometry (ToF-SIMS) and immunostaining. Principle component analysis (PCA) of the ToF-SIMS spectra and quantitative immunofluorescence data revealed distinct differences and trends in the complex ECM composition. The introduced methodology is validated by cell culture experiments using the decellularized matrix variants and concluded to provide a new level of control in tailoring matrix properties for tissue and organoid models (authors MN and VM contributed equally).