| AVS 66th International Symposium & Exhibition | |
| Biomaterial Interfaces Division | Tuesday Sessions |
| Session BI+AS-TuM |
| Session: | Characterization of Biological and Biomaterial Surfaces |
| Presenter: | Yuchen Zhang, Pacific Northwest National Laboratory |
| Authors: | Y.C. Zhang, Pacific Northwest National Laboratory X.-Y. Yu, Pacific Northwest National Laboratory |
| Correspondent: | Click to Email |
This presentation aims to show that we have successfully used delayed image extraction in time-of-flight secondary ion mass spectrometry (ToF-SIMS) to study the interaction between Brachypodium seed and plant growth-promoting bacteria (PGPB) for the first time.
Abstract
The use of time-of-flight secondary ion mass spectrometry (ToF-SIMS) is a relatively unexplored in plant biology that is undergoing fast development [1]. The majority of existing research in plant biology that has utilized ToF-SIMS mostly involves the study of plant stems and leaves, and only a handful of studies apply it on the analysis of plant roots and/or seeds. Additionally, the use of Brachypodium distachyon (Brachypodium) as a model system for research has become more prominent in plant biology. Brachypodium, a C3 model, can provide more insight into the biological studies of other species including small grain crops such as wheat and barley [2], therefore it has a significant scientific impact in plant biology. To the best of our knowledge, this will be the first systematic ToF-SIMS imaging of Brachypodium. In this work, we obtained chemical mapping of the interaction of grains of Brachypodium with plant growth-promoting bacteria (PGPB) [3], namely, Pseudomonas and Arthrobacter, using ToF-SIMS. Specifically, the use of the delayed image extraction mode in ToF-SIMS provides chemical speciation of the Brachypodium seed surface and simultaneously captures the morphological features of the plant-bacteria interface. Our findings provide high resolution spatial distributions of fatty acids (e.g., palmitic acid, stearic acid, and arachidic acid) and phospholipid (e.g., cardiolipin) present on the Brachypodium seed surface. Spectral PCA results indicate that the biofilm and planktonic cells both have effects on the seed surfaces. In terms of seedling potentials, the seed brush is the most active after PGPB attachment on the biointerface.
Key words: ToF-SIMS, delayed image extraction, PGPB, Brachypodium, Pseudomonas, Arthrobacter
References:
1. Boughton, B.A., et al., Mass spectrometry imaging for plant biology: a review. Phytochem Rev, 2016. 15: p. 445-488.
2. Delaplace, P., et al., Influence of rhizobacterial volatiles on the root system architecture and the production and allocation of biomass in the model grass Brachypodium distachyon (L.) P. Beauv. BMC Plant Biol, 2015. 15: p. 195.
3. Scholthof, K.B.G., et al., Brachypodium: A Monocot Grass Model Genus for Plant Biology. Plant Cell, 2018. 30(8): p. 1673-1694.