AVS 66th International Symposium & Exhibition
    Biomaterial Interfaces Division Tuesday Sessions
       Session BI+AS-TuA

Paper BI+AS-TuA9
The Hybrid Nano-biointerfaces Between Gold, Graphene Oxide and Angiogenin for Wound Repair

Tuesday, October 22, 2019, 5:00 pm, Room A120-121

Session: Biomolecules and Biophysics and Interfaces & Flash Session
Presenter: Diego La Mendola, University of Pisa, Italy
Authors: D. La Mendola, University of Pisa, Italy
L.M. Cucci, University of Catania, Italy
G. Villaggio, University of Catania, Italy
C. Satriano, University of Catania, Italy
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Angiogenin (ANG) is a member of the ribonuclease family and a physiological constituent of the human plasma. Ang is a potent angiogenic factor regulating a wide range of responses, such as angiogenesis, cell proliferation, cell migration, and pro-survival effects. ANG has been shown related to many pathophysiological processes, including cancers, neurodegeneration, inflammation and regeneration of damaged tissues. In this work, we investigated a hybrid obtained by the assembly of ANG to gold nanoparticles and graphene oxide nanosheets, to exploit the synergic effects of antioxidant AuNP and antimicrobial GO, respectively. Au-GO-ANG were characterized by UV-visible spectroscopy, to correlate the changes in the plasmonic peak as well as in the π-->π* transitions to the protein interaction with Au and GO, respectively. QCM-D measurements on supported lipid bilayers, as model of cell membranes, pointed to a stronger interaction of the AuNP-Ang systems in comparison with the uncoated nanoparticles. The developed systems promoted fibroblasts migration and wound closure. Confocal microscopy cell imaging evidenced dynamic processes at the level of cytoskeleton and sub-cellular compartments. The results reveal a promising multifunctional platform for wound care treatment and tissue regeneration.