Paper BI+AS+NS-MoM11
Biosensing Applications of Silver Nanorods Array Fabricated by Glancing Angle Deposition (GLAD)

Monday, October 21, 2019, 11:40 am, Room A120-121

Silver being most widely used material due to its unique electrical and optical properties. Here we have investigated biosensing properties of silver nanorods array (AgNRs) fabricated by glancing angle deposition. GLAD grown silver nanorods are found to be highly sensitive and selective for hydrogen sulfide (H₂S) gas. Color and water wetting (contact angle) of AgNRs array are parameters affected in the presence of this gas. H₂S is one the major gaseous products evolved in bacterial metabolic process. On the basic of H₂S production, we have shown the detection of viability as well as antibiotic resistance in different strains of bacteria.

Another potential application of as synthesized AgNRs array in Surface enhanced Raman scattering (SERS) based detection. The dengue is a viral disease and a serious global health concern. About 2.5 billion of world’s population has been living at the risk of dengue infection. It causes a spectrum of illness ranges from acute febrile illness called dengue fever (DF) to more severe life threatening forms dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS) causing vascular leakage that may lead to death. So far, neither specific treatment nor effective vaccine available for the prevention and treatment. Therefore, early detection is the key of the survival of the patients. The earlier symptom starts with mild dengue fever, at this stage the concentration of the biomarkers are very less which pose a problem in early detection. In the present study, we have demonstrated the detection of dengue from clinical blood samples employing AgNRs array as SERS substrates with hand held Raman spectrometer. A notable change in SERS spectral signature observed in the blood of dengue infected patients in comparison to that of healthy subject. This change was further confirmed using the statistical tool principal component analysis (PCA) and the samples were differentiated as healthy, dengue positive and dengue negative. All the blood samples were also dually verified with Antigen (NS1) as well as Antibody (IgM) ELISA kit. This method provides a field deployable, rapid diagnosis of dengue at its early stage.