| AVS 66th International Symposium & Exhibition | |
| Biomaterial Interfaces Division | Monday Sessions |
| Session BI+AS+NS-MoM |
| Session: | Biofabrication, Bioanalytics, Biosensors, Diagnostics, Biolubrication and Wear |
| Presenter: | Ridwan Fayaz Hossain, University of North Texas |
| Authors: | R.F. Hossain, University of North Texas A.B. Kaul, University of North Texas |
| Correspondent: | Click to Email |
Protein detection biosensors are interesting tools for detecting and measuring the levels of specific proteins in biological and environmental samples, offering certain operational advantages over standard photometric methods, notably with respect to rapidity, ease-of-use, cost, simplicity, portability, and ease of mass manufacture. Although inkjet printed electrode based sensor is widely reported, the number of fully inkjet printed biosensors is still limited [1,2]. Here, we report the design, fabrication, and evaluation of a flexible field-effect transistor (FET) for biosensing based on the inkjet printing technique, where the insulator layer is uniquely functionalized for Immunoglobulin G (IgG) protein detection. IgG is a plasma-cell protein that is produced within the lymph nodes, spleen, bone marrow, respiratory tract mucosa, tissue, etc. Since IgG protein is produced as part of the body's response to bacteria, viruses, and tissue antigens, measurement of blood IgG levels can reveal any of the body’s abnormal conditions. Until now, proteins are detected mostly by antibodies in analytical formats like ELISA, immunobead assay, western blotting, and microarrays, etc. but their performance is limited by low sensitivity. This new generation biosensor is more stable and well adapted to the conditions of real samples. The protein detection biosensor reported here represents an important starting point for the design and fabrication of flexible, rapid detection biosensing devices by inkjet printing. This work shows a promising aspect of protein detection that will pave the way for the development of a fully functional device for point-of-care diagnosis.
Reference:
[1] Jensen, G. C., Krause, C. E., Sotzing, G. A., & Rusling, J. F. (2011). Inkjet-printed gold nanoparticle electrochemical arrays on plastic. Application to immunodetection of a cancer biomarker protein. Physical Chemistry Chemical Physics, 13(11), 4888-4894.
[2] Carvajal, S., Fera, S. N., Jones, A. L., Baldo, T. A., Mosa, I. M., Rusling, J. F., & Krause, C. E. (2018). Disposable inkjet-printed electrochemical platform for detection of clinically relevant HER-2 breast cancer biomarker. Biosensors and Bioelectronics, 104, 158-162.