| AVS 66th International Symposium & Exhibition | |
| 2D Materials | Wednesday Sessions |
| Session 2D+EM+MN+NS-WeA |
| Session: | 2D Device Physics and Applications |
| Presenter: | Sebastian Hentz, CEA-LETI, France |
| Authors: | A. Hugo, CEA-LETI, France C. Sun, Northwestern University M. Kumar, CEA-LETI, France R. Othmen, CNRS-Institut Néel, France J. Renard, CNRS-Institut Néel, France V. Bouchiat, CNRS-Institut Néel, France J. Mann, Northwestern University J.M. Parpia, Cornell University H.G. Craighead, Cornell University P. Mailley, CEA-LETI, France W.R. Dichtel, Northwestern University T. ALAVA, CEA-LETI, France Hentz, CEA-LETI, France |
| Correspondent: | Click to Email |
Electrical detection is a very robust technique to transduce the adsorption of charged protein to a biological selective layer (i.e. biosensing). Electrolyte gated field effect transistors (EGFET) integrating graphene monolayers as the transducing element have shown outstanding electrical sensitivity in liquid compared to silicon and diamond based EGFET. In order to build graphene EGFET as effective biosensing unit it is important to attach at its surface a functional layers of biological molecules that will carry the task of enforcing specific detection of compound. Protein are widely used as specific bioreceptor for sensor biological functionalization yet it has been shown that protein lose their function when simply adsorbed on graphene. Covalent binding being out of the way for 2D dimensional crystals such as graphene (for the inherent deterioration of mechanical and electrical properties) we have shown that custom made tripodal compound attaching the graphene basal plane through Pi-stacking of aromatic moieties could be used to attach specific biomolecules to graphene while maintaining their biological function hence their specificity. In this report we present an optimized fabrication process for graphene EGFET that includes patterning and passivation of electrical contact. The devices reproducibly show state of the art electrical performances. We demonstrate that the process can be simply transferred to different host substrates to integrate graphene EGFET ubiquitously on Silicon, glass or printed circuit board with similar performances. Finally, we implemented biological functionalization of the sensors by attaching streptavidin to the sensor thanks to the non-covalent tripodal compound. We report consistent changes in the Dirac peak of graphene due to the adsorption of tripodal compound and streptavidin as well as the binding of biotin, specifically bound to streptavidin. We show the detection to be specific and reproducible.