| AVS 65th International Symposium & Exhibition | |
| Biomaterial Interfaces Division | Wednesday Sessions |
| Session BI-WeA |
| Session: | Microbes and Fouling at Surfaces |
| Presenter: | Camilo Jaramillo, University of Illinois at Urbana-Champaign |
| Authors: | C. Jaramillo, University of Illinois at Urbana-Champaign A.F. Civantos, University of Illinois at Urbana-Champaign J.P. Allain, University of Illinois at Urbana-Champaign |
| Correspondent: | Click to Email |
First reported in the late 1950s, antibiotic-resistant bacteria have become an issue of major concern 1. This has motivated the study of other mechanisms to provide interfaces with antibacterial activity, including surface chemistry, surface topography and other physicochemical properties 2. Among these mechanisms, the physico-mechanical effects have also attracted attention. An example of this concept can be found in natural nanostructured surfaces. The nanopatterned surface of the cicada wings has been observed to possess very effective bactericidal activity, via a chemistry-independent mechanism 3. Chitosan, a biodegradable and non-toxic biopolymer with antibacterial properties, has been used for wound treatment, drug delivery and biosensing applications 4. These properties make it an attractive material to be used in biointerfaces. Following the same concept of the cicada wings, nanopatterned silicon surfaces coated with CS showed enhanced antibacterial activity, when compared to un-coated Si surfaces 5.
In previous works from our group, we had shown that Directed Plasma Nanosynthesis (DPNS) can induce the formation of nanofeatures on the surface of chitosan. In this work, we further study the effects of DPNS on the formation of nanopatterns on the surface of different chitosan membranes are further studied, using angle of irradiation as a control parameter. Additionally, the biocidal action of the modified surfaces is studied by running in vitro tests with E. coli. SEM images were used to evaluate the nanofeatures induced on the surfaces, as well as their effects on the incubated bacteria. Studying the antibacterial activity of these nanopatterned surfaces constitutes a step towards elucidating the mechanisms of antibacterial activity based on physico-mechanical effects.
References:1. Levy, S. B. & Bonnie, M. Antibacterial resistance worldwide: Causes, challenges and responses. Nature Medicine10, S122–S129 (2004).
2. Bazaka, K. et al. Anti-bacterial surfaces: natural agents, mechanisms of action, and plasma surface modification. RSC Adv.5, 48739–48759 (2015).
3. Ivanova, E. P. et al. Natural bactericidal surfaces: Mechanical rupture of pseudomonas aeruginosa cells by cicada wings. Small8, 2489–2494 (2012).
4. Bano, I., Arshad, M., Yasin, T., Ghauri, M. A. & Younus, M. Chitosan: A potential biopolymer for wound management. International Journal of Biological Macromolecules102, 380–383 (2017).
5. Tripathy, A. et al. Impact of Bioinspired Nanotopography on the Antibacterial and Antibiofilm Efficacy of Chitosan. Biomacromolecules acs.biomac.8b00200 (2018). doi:10.1021/acs.biomac.8b00200