AVS 65th International Symposium & Exhibition
    Biomaterial Interfaces Division Tuesday Sessions
       Session BI-TuP

Paper BI-TuP4
Fluorescent DNA Nanosphere Barcdoe System by Rolling Circle Amplification for Tumor Cells Detection

Tuesday, October 23, 2018, 6:30 pm, Room Hall B

Session: Biomaterial Interfaces Division Poster Session
Presenter: JongBum Lee, University of Seoul, Republic of Korea
Authors: SW. Han, University of Seoul, Republic of Korea
JB. Lee, University of Seoul, Republic of Korea
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Nucleic acid-based nanotechnologies have been developed with the base pairing property and applied to numerous bioengineering fields of study. As a novel engineering material, DNA has been used to fabricate nanostructures from simple 2D structures to complex 3D structures. With this development of DNA nanoarchitecture, enzymatic replication technique has been also attracted as a new strategy for building nucleic acid-based nanostructures. Here, DNA nanosphere (DNANS) is fabricated by rolling circle amplification (RCA) and coated with antibodies for target cells detection. DNANS can be applied as a barcode system which can distinguish the tumor cells by recognizing tumor-specific protein. As a proof of concept, the capability for target detection of DNANS barcode system was demonstrated. This target-specific antibody-coated DNANS suggests a new route for the simple and selective recognition of cancer cells.