| AVS 65th International Symposium & Exhibition | |
| Biomaterial Interfaces Division | Tuesday Sessions |
| Session BI-TuP |
| Session: | Biomaterial Interfaces Division Poster Session |
| Presenter: | Jinyeong Kim, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Republic of Korea |
| Authors: | O.S. Kwon, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Republic of Korea J.Y. Kim, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Republic of Korea S.J. Park, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Republic of Korea |
| Correspondent: | Click to Email |
Dopamine (DA) has been studied in the field of nervous and cardiovascular systems. Abnormal levels of dopamine is an indicator of neurological disorders, resulting in Alzheimer's and Parkinson's diseases. Therefore, dopamine is a clinically useful diagnostic sign and requires a novel approach with high sensitivity, selectivity and a rapid response. Various sensors have been developed, such as high-performance liquid chromatography (HPLC), mass spectroscopy, and spectrophotometry. However, they are limited by their high cost, low sensitivity, and variable label response.
The field-effect transistor (FET) has been used in the development of diagnosis for several decades. It is gated by changes of charge carrier density in the channel induced by the binding of target molecules, leading to high-performance biosensors. In addition, the FET platform has attracted due to their low cost, easy operation, fast response, label-free operation, parallel sensing as well as high sensitivity.
In this article, we introduced a high performance dopamine sensor based on FET assay. Multidimensional carboxylated poly(3,4-ethylenedioxythiophene) (MCPEDOT) NFs membrane was utilized as the conductive channel of sensor in the FET system. Interestingly, it provided high performance sensing due to enhanced interaction from high surface area and gate-potential modulators. Moreover, hDRD1, G protein-coupled receptors (GPCRs) as the recognition elements, was first expressed in Escherichia coli and modified with the surface of MCPEDOT NFs, leading to high selectivity. As a results, the hDRD1−MCPEDOT NF-based FET exhibits a rapid real-time response (<2 s) with high dopamine selectivity and sensitivity performance (approximately 100 fM).