| AVS 65th International Symposium & Exhibition | |
| Biomaterial Interfaces Division | Thursday Sessions |
| Session BI-ThM |
| Session: | Biomolecules and Biophysics at Interfaces |
| Presenter: | Diego La Mendola, University of Pisa, Italy |
| Authors: | I. Naletova, University of Catania, Italy L.M. Cucci, University of Catania, Italy F. D'Angeli, University of Catania, Italy C.D. Anfuso, University of Catania, Italy G. Lupo, University of Catania, Italy A. Magrì, National Council of Research (CNR), Italy C. Satriano, University of Catania, Italy D. La Mendola, University of Pisa, Italy |
| Correspondent: | Click to Email |
In this work, hybrid assemblies of plasmonic gold nanoparticles (AuNPs) and peptides mimicking the putative cell binding domain of angiogenin protein (60-68 sequence)1 were investigated in their interaction with artificial membranes of supported lipid bilayers (SLBs) and cellular membranes of cancer cell lines. In particular, the response of glioblastoma cell line (A172), as model of the most aggressive cancer that begins within the brain2, and neurons obtained by differentiated neuroblastoma cell line (SHSY5Y), as ‘normal cells’, was scrutinized. The influence of copper, which is a pivotal co-player of cellular homeostasis in both physiological and pathological angiogenesis, was investigated in parallel with the gold nanoparticles functionalized with a fluorescent derivative of Ang(60-68) peptide. Control experiments using the non-fluorescent peptide analogous immobilized onto the AuNPs either by physisorption (Ang(60-68)) or chemisorption (Ang(60-68)Cys) were also included. The hybrid peptide/AuNP/copper systems were characterized by means of UV-visible, AFM and CD, to address the plasmonic changes, the nanoparticle coverage and conformational features at the hybrid biointerface. Lateral diffusion measurements on SLBs after their interaction with the peptide-functionalised AuNPs pointed to a stronger membrane interaction in comparison with the uncoated nanoparticles. Cell viability and proliferation assays indicated significant differences, in the presence or absence of copper, for the two cell lines. Cell imaging by confocal microscopy evidenced dynamic processes modulated in a synergic way by the different components (peptide, gold nanoparticle, copper) of the hybrid nanoplatforms at the level of the cell membrane (cytoskeleton features observed by actin staining) as well as at the sub-cellular compartments (copper-binding proteins).
[1] Cucci LM, Munzone A, Naletova I, Magrì A, La Mendola D, Satriano C. Biointerphases. 2018;13(3):03C401.
[2] Bleeker FE, Molenaar RJ, Leenstra S. Recent advances in the molecular understanding of glioblastoma.J Neurooncol. 2012;108(1):11-27.