| AVS 65th International Symposium & Exhibition | |
| Biomaterial Interfaces Division | Thursday Sessions |
| Session BI-ThM |
| Session: | Biomolecules and Biophysics at Interfaces |
| Presenter: | Cristina Satriano, University of Catania, Italy |
| Authors: | V.C.L. Caruso, University of Catania, Italy G. Trapani, University of Catania and Scuola Superiore di Catania, Italy L.M. Cucci, University of Catania, Italy I. Naletova, University of Catania, Italy D. La Mendola, University of Pisa, Italy C. Satriano, University of Catania, Italy |
| Correspondent: | Click to Email |
Graphene oxide (GO) nanosheets, owing to their high surface-to-volume ratio and the richness of oxygen-containing moieties (including carboxyl, hydroxyls and epoxide groups) represent ideal 2D nanoplatforms for drug delivery applications [1]. The integration of GO with homo-aromatic dipeptides, which are able to self-assemble into ordered structures such as nanotubes and nanowires [2], may offer unique potentialities at the biointerface because of the increased biocompatibility of the hybrid system and, remarkably, for the capability to load/protect a cargo into the peptide nanocontainers. Moreover, metal ions can influence/drive the peptide self-assembling process as well as to induce additional properties of the hybrid system (e.g., antibacterial and angiogenic properties in the case of Cu2+ions [3]).
In this work, the hydrophobic dipeptides Phe-Phe (FF) and Tyr-Tyr (YY) were grown in the presence of graphene oxide (GO) and copper ions, to fabricate hybrid peptide-GO-metal nanoassemblies with multifaceted features.
The nanoassemblies were scrutinised spectroscopically (UV-visible, fluorescence and circular dichroism) and microscopically (atomic force microscopy and confocal microscopy). Quartz crystal microbalance with dissipation monitoring (QCM-D) was used for real-time acoustic sensing of the interaction of the hybrid nanoplatforms with supported lipid bilayers, used as model cell membranes. Promising results of cellular uptake in neuroblastoma cells were measured by confocal microscopy for the assemblies loaded with the anticancer drug doxorubicine.
[1] Consiglio, G., Di Pietro, P., D'Urso, L., Forte, G., Grasso, G., Sgarlata, C., ... & Satriano, C. (2017). Surface tailoring of polyacrylate-grafted graphene oxide for controlled interactions at the biointerface. Journal of colloid and interface science, 506, 532-542.
[2] Reches, M., & Gazit, E. (2003). Casting metal nanowires within discrete self-assembled peptide nanotubes. Science, 300(5619), 625-627.
[3] Yu, L., Jin, G., Ouyang, L., Wang, D., Qiao, Y., & Liu, X. (2016). Antibacterial activity, osteogenic and angiogenic behaviors of copper-bearing titanium synthesized by PIII&D. Journal of Materials Chemistry B, 4(7), 1296-1309.