| AVS 65th International Symposium & Exhibition | |
| Biomaterial Interfaces Division | Tuesday Sessions |
| Session BI+AS+IPF+NS-TuA |
| Session: | IoT Session: Biofabrication, Bioanalytics, Biosensors and Diagnostics and Flash Networking Session |
| Presenter: | Riley Bednar, Department of Biochemistry and Biophysics, Oregon State University |
| Authors: | R.M. Bednar, Department of Biochemistry and Biophysics, Oregon State University R.A. Mehl, Department of Biochemistry and Biophysics, Oregon State University |
| Correspondent: | Click to Email |
The functionalization of material surfaces with proteins is of great importance to a number of technologies, from industrial processes to biomedical diagnostics. However, while it has been proposed that orientation may be important to the function of such biomaterials, efforts to study such roles are hampered by a lack of rapid, quantitative, and orientation-specific immobilization techniques which will reduce non-specific fouling, and allow substoichiometric attachment of proteins onto surfaces in an orientation-controlled manner. Here, Carbonic Anydrase II (HCA)—a 30 kDA, monomeric metalloenzyme which catalyzes the interconversion of carbon dioxide to bicarbonate—is immobilized onto strained trans-cyclooctene (sTCO)-functionalized magnetic resin in an orientation-specific manner via bioorthogonal ligation with a site-specifically installed tetrazine-containing amino acid (Tet2.0).