| AVS 65th International Symposium & Exhibition | |
| Biomaterial Interfaces Division | Tuesday Sessions |
| Session BI+AS+IPF+NS-TuA |
| Session: | IoT Session: Biofabrication, Bioanalytics, Biosensors and Diagnostics and Flash Networking Session |
| Presenter: | Johannes Daniel Bartl, Walter Schottky Institut (WSI) and Physics Department, Technische Universität München, Germany |
| Authors: | J.D. Bartl, Walter Schottky Institut (WSI) and Physics Department, Technische Universität München, Germany P. Scarbolo, Dipartimento Politecnico di Ingegneria e Architettura (DPIA), Università degli Studi di Udine, Italy S. Gremmo, Walter Schottky Institut (WSI) and Physics Department, Technische Universität München, Germany G. Rziga, Walter Schottky Institut (WSI) and Physics Department, Technische Universität München, Germany M. Stutzmann, Walter Schottky Institut (WSI) and Physics Department, Technische Universität München, Germany M. Tornow, Molecular Electronics Group and Department of Electrical and Computer Engineering, Technische Universität München, Germany L. Selmi, Dipartimento di Ingegneria "Enzo Ferrari" (DIEF), Università di Modena e Reggio Emilia, Italy A. Cattani-Scholz, Walter Schottky Institut (WSI) and Physics Department, Technische Universität München, Germany |
| Correspondent: | Click to Email |
Silicon-based field-effect devices have been widely studied for label-free DNA detection in recent years. These devices rely on the detection of changes in the electrical surface potential during the DNA recognition event and thus require a reliable and selective immobilization of charged biomolecules on the device surface [1]. The preparation of self-assembled monolayers of phosphonic acids (SAMPs) on metal oxide surfaces is an efficient approach to generate well-defined organic interfaces with a high density of receptor binding sites close to the sensing surface [2,3]. In this work, we report the functionalization and characterization of silicon/silicon nitride surfaces with different types of peptide nucleic acid (PNA), a synthetic analogue to DNA [4].
Differently modified PNA molecules are covalently immobilized on the underlying SAMPs either in a multidentate or monodentate fashion to investigate the effect of different binding modes on receptor density and morphology important for PNA-DNA hybridization. Multidentate immobilization of the bioreceptors via C6-SH attachment groups at the γ-points along the PNA backbone provides a rigid, lying configuration on the device surface (PNA 1), whereas a monodentate immobilization by Cys-capped PNA molecules (PNA 2) results in more flexible and more accessible receptor binding sites. Our results indicate that the presented functionalization scheme can be successfully applied to produce morphologically and electrochemically different PNA bioreceptor binding sites on silicon/silicon nitride surfaces. Consequently, a well-chosen modification of the PNA backbone is a valid approach to influence the sensing properties of surface-immobilized PNA bioreceptors, which might provide an additional parameter to further tune and tailor the sensing capabilities of PNA-based biosensing devices.
[1] Ingebrandt S. and Offenhausser A., Phys. Status Solidi A 203 (2006), 3399–3411.
[2] Chaki N. K. and Vijayamohanan K., Biosens. & Bioelectron. 17 (2002), 112.
[3] Stutzmann M., Garrido J. A., Eickhoff M. and Brandt M. S., Phys. Status Solidi A 203 (2006), 3424–3437.
[4] Nielsen P. E. and Egholm M. (ed.), Peptide Nucleic Acids, Horizon Scientific Press (1999).