| AVS 65th International Symposium & Exhibition | |
| Biomaterial Interfaces Division | Monday Sessions |
| Session BI+AS+IPF+MN-MoA |
| Session: | Advanced Imaging and Structure Determination of Biomaterials Research |
| Presenter: | Xiao-Ying Yu, Pacific Northwest National Laboratory |
| Authors: | X-Y. Yu, Pacific Northwest National Laboratory R. Komorek, Pacific Northwest National Laboratory Z.H. Zhu, Pacific Northwest National Laboratory C.J. Jansson, Pacific Northwest National Laboratory |
| Correspondent: | Click to Email |
We present the first imaging and spectra results of plant root interactions with plant growth-promoting bacteria (PGPB) using time-of-flight secondary ion mass spectrometry (ToF-SIMS), showing the successful application of delayed image extraction to study plant biology. Compared to MALDI (Matrix Assisted Laser Desorption Ionization), an imaging mass spectrometry technique widely used in plant studies,[1] SIMS is less destructive and provides submicrometer spatial mapping of molecular species of importance in metabolic processes. Brachypodium distachyon (Brachypodium), a genomics model for bioenergy and native grasses, is used due to its small diploid genome, close phylogenetic links to other grass species, relative ease of genetic transformation, short life cycle, small stature, and simple growth requirements.[2] Plant growth-promoting bacteria (PGPB) such as Pseudomonas and Arthrobacter were introduced to Brachypodium roots prior to analysis, and their potential effect on root extrusion was studied using ToF-SIMS imaging. Specifically, delayed image extraction was used in data acquisition. This approach was chosen to obtain high mass and high spatial resolutions.[3] Excellent SIMS imaging gives topographical description of the root surface with and without PGPB interactions. Distinctive characteristic peaks are observed, indicating compositional changes with and without PGPB introduction to the root surface beside visible surface morphological variations. Our initial results demonstrate that ToF-SIMS is a promising imaging mass spectrometry tool to study plant biology and root-microbe interactions and provide molecular-level insight at the biointerface with high spatial resolution.
References:
[1] D Sturtevant et al., Three-dimensional visualization of membrane phospholipid distributions in Arabidopsis thaliana seeds: A spatial perspective of molecular heterogeneity, Biochimica et Biophysica Acta (2017),1862(2), 268-81.
[2] T Girin et al., Brachypodium: a promising hub between model species and cereals, J. Experimental Botany (2014),65(19), 5683-96.
[3] QP Vanbellingen et al., Time-of-flight secondary ion mass spectrometry imaging of biological samples with delayed extraction for high mass and high spatial resolutions, Rapid Comm. Mass Spectrom. (2015), 29 (13), 1187-95.