AVS 64th International Symposium & Exhibition
    Plasma Processing for Biomedical Applications Focus Topic Tuesday Sessions
       Session PB+BI+PS-TuM

Paper PB+BI+PS-TuM4
Selective Antitumor Effect of the Plasma-Activated Medium Produced by Atmospheric Pressure Plasma with High Plasma Density

Tuesday, October 31, 2017, 9:00 am, Room 12

Session: Plasma Medicine
Presenter: Yohei Takahashi, Nikon Corporation, Japan
Authors: Y. Takahashi, Nikon Corporation, Japan
Y. Taki, Nikon Corporation, Japan
K. Takeda, Meijo University, Japan
H. Hashizume, Nagoya University, Japan
H. Tanaka, Nagoya University, Japan
M. Hori, Nagoya University, Japan
Correspondent: Click to Email
Recently, atmospheric pressure plasma has been widely developed for the applications on various fields, such as synthesis approaches, surface modification, sterilization, etc. Especially, cancer therapy using atmospheric pressure plasma is one of the most attractive applications. The culture medium irradiated with the atmospheric pressure plasma was called Plasma-Activated Medium (PAM), which exhibited the selective apoptotic cell death of cancer cells. In this study, we have demonstrated the antitumor effect of medium induced by irradiation of atmospheric pressure plasma with high plasma density and compared the cell survival between cancer and normal cells, which showed that the selective apoptotic cell death was achieved. Additionally, the basic diagnostics of the plasma and the analysis of the PAM were performed and the relation with the antitumor effects was discussed. The emission peak of OH radical (A2Σ-X2Π) was observed in the atmospheric pressure plasma. This transition is the intense systems emitted by low temperature plasmas containing even a small amount of H2O. The selective apoptotic cell death effect by treatment with PAM produced by atmospheric pressure plasma irradiation was confirmed. The survival of cancer cell after incubation in PAM was greatly lower than that of normal cell was. The productions of H2O2 and NO2- by irradiation of high density plasma were detected by the colorimetric assay. The synergistic effect of H2O2 and NO2- in PAM is considered to affect the proliferation of cancer cells.