| AVS 63rd International Symposium & Exhibition | |
| Novel Trends in Synchrotron and FEL-Based Analysis Focus Topic | Tuesday Sessions |
| Session SA+AS+BI+MI-TuA |
| Session: | Synchrotron and XFEL Advances for Biological Systems (2:20-3:40 pm)/Synchrotron Radiation at the Frontiers of Device Technology (4:20-6:20 pm) |
| Presenter: | Pupa Gilbert, University of Wisconsin - Madison |
| Correspondent: | Click to Email |
X-ray linear dichroism was first shown in natural biominerals by Metzler et al. [1]. Based on this effect, we developed Polarization-dependent Imaging Contrast (PIC)-mapping, which displayed non-quantitative crystal orientation at the nanoscale as gray levels in ratios of images acquired at different linear polarizations [2]. A later development provided grayscale, semi-quantitative PIC-maps by acquiring stacks of 19 images as the linear polarization was rotated in 5° intervals from 0° to 90° [3-7]. The latest development uses the same stacks of images to fully, quantitatively display crystal orientations in colors, including hue and brightness, which represent in-plane and off-plane crystallographic c-axis orientation angles [8-10].
Using PIC-mapping in these 3 subsequent modes, we discovered several biomineral formation mechanisms in nacre [11,7], sea urchin teeth [12-14], ascidian spicules [10], corals, eggshells, modern and fossil sea shell ultrastructure [15].
1. RA Metzler et al., Phys. Rev. Lett. 98, (2007). DOI: http://dx.doi.org/10.1103/PhysRevLett.98.268102
2. RA Metzler et al., Phys Rev B 77, 064110-1, (2008). DOI: http://dx.doi.org/10.1103/PhysRevB.77.064110
3. PUPA Gilbert et al., Proc Natl Acad Sci USA 108, (2011). DOI: 10.1073/pnas.1107917108
4. PUPA Gilbert, J Electr Spectrosc Rel Phenom, special issue on Photoelectron microscopy, Time-resolved pump-probe PES 185, (2012). DOI: http://dx.doi.org/10.1016/j.elspec.2012.06.001
5. IC Olson et al., J Am Chem Soc 134, (2012. JOURNAL COVER). DOI: dx.doi.org/10.1021/ja210808s
6. IC Olson et al., J Struct Biol 183, (2013). DOI: 10.1016/j.jsb.2013.06.006
7. IC Olson et al., J Struct Biol 184, (2013. JOURNAL COVER). DOI: 10.1016/j.jsb.2013.10.002
8. RT DeVol et al., J Phys Chem B 118, (2014). DOI: 10.1021/jp503700g
9. RT DeVol et al., J Am Chem Soc 137, (2015). DOI: 10.1021/jacs.5b07931
10. B Pokroy et al., Chem Mater 27, (2015. JOURNAL COVER.). DOI: 10.1021/acs.chemmater.5b01542
11. PUPA Gilbert et al., J Am Chem Soc 130, 17519, (2008). DOI: 10.1021/ja8065495
12. CE Killian et al., J Am Chem Soc 131, (2009). DOI: 10.1021/ja907063z
13. YR Ma et al., Procs Natl Acad Sci USA 106, (2009). DOI: 10.1073/pnas.0810300106
14. CE Killian et al., Adv Funct Mater 21, (2011). DOI: 10.1002/adfm.201001546
15. PUPA Gilbert et al., in preparation, (2016).