| AVS 63rd International Symposium & Exhibition | |
| Plasma Processing for Biomedical Applications Focus Topic | Tuesday Sessions |
| Session PB+BI+PS-TuM |
| Session: | Plasma Processing of Biological/Biomimetic Surfaces |
| Presenter: | Jaroslav Kristof, Shizuoka University, Japan |
| Authors: | J. Kristof, Shizuoka University, Japan N. Tran, Shizuoka University, Japan M. Blajan, Shizuoka University, Japan K. Shimizu, Shizuoka University, Japan |
| Correspondent: | Click to Email |
Application of drugs by needles presents risk of infections and causes pain. On the other side, oral application of drugs can be toxic for human body because drug has to be transported through alimentary tract and higher amount of active agent is required. Transdermal delivery could be ideal painless and effective way but barrier function of skin has to be reduced for improving permeability of drugs. Research of last years proves that plasma can interact with skin and cause decreasing barrier function of skin [1-3].
We used plasma jet and microplasma discharge for investigation of barrier function of stratum corneum – horny layer of of Yucatan micropig skin. Helium or argon was used as working gas. These rare gases were later enriched by liquids like water or ethanol through the bubbling system to achieve higher amount of active particles like OH.
Physical changes of the pig skin were observed by microscope. As the human body is not non-conductive, we can expect different results when conductivity of layer under skin is changed. We compared effect of plasma on conductive and non-conductive material. Placement of skin on conductive material caused burned spots on skin by plasma jet [3]. While it was isolated, no damage was observed with plasma jet irradiation. In case of treatment of skin by microplasma, physical damage was hardly observed.
Changes in stratum corneum layer were observed by Attenuated Total Reflectance – Fourier Transform InfraRed (ATR-FTIR) spectroscopy. ATR-FTIR spectrum offer information about water, lipid bilayer and proteins in stratum corneum. Permeability of skin for drugs correlates with shift of asymmetric stretch of CH2 band to higher wavenumbers. This information describes behavior of lipid bilayer. Information about reaction of proteins on plasma treatment content Amide I and Amide II bands. Reaction of stratum corneum layer of pig skin depended on used discharge type and gas. Effectivity of plasma sources and used gases or gas mixtures for transdermal drug delivery was analysed.
References
[1] Lademann J., A Patzelt A., Richter H., Lademann O., Baier G., Breucker L., Landfester K., Laser Phys. Lett.10, 083001 (2013).
[2] Wu A. S. et. al., Journal of surgical research179(1), E1-E12 (2013).
[3] Shimizu K., Hayashida K. and Blajan M, Biointerphases10(2), 029517 (2015).