AVS 63rd International Symposium & Exhibition
    Plasma Processing for Biomedical Applications Focus Topic Monday Sessions
       Session PB+BI+PS-MoA

Invited Paper PB+BI+PS-MoA8
Low and Atmospheric Pressure Plasma Polymerization for Immunosensing and Tissue Engineering

Monday, November 7, 2016, 4:00 pm, Room 101A

Session: Plasma Processing of Biomaterials
Presenter: Lenka Zajickova, Masaryk University, Czech Republic
Authors: L. Zajickova, Masaryk University, Czech Republic
A. Manakhov, Masaryk University, Czech Republic
E. Makhneva, Masaryk University, Czech Republic
J. Medalova, Masaryk University, Czech Republic
D. Necas, Masaryk University, Czech Republic
L. Strbkova, Brno University of Technology, Czech Republic
A. Obrusnik, Masaryk University, Czech Republic
M. Landova, Masaryk University, Czech Republic
Correspondent: Click to Email
Plasma polymerization provides a large playground for the preparation of surfaces suitable for immobilization of biomolecules and colonization by cells because chemical, structural and functional properties of plasma polymerized thin films can be tuned accordingly. The key decision for the particular application is the selection of functional chemical group that the final plasma polymer should contain. This contribution is going to discuss deposition of plasma polymers containing amine and carboxyl groups, functional groups that are typically used in biochemical applications and that are proposed to influence positively the attachment and proliferation of cells at surfaces. Amine-rich films were deposited in the low pressure pulsed radio frequency discharge using vapors of cyclopropylamine mixed with argon. The films contained primary and secondary amines and a small amount of oxygen. The structure of the films, reflected in their stability in water, could be tuned by the plasma conditions. The relationship between the amount of amine groups and the water stability was not straitforward because the films with similar amount of primary amine groups but different cross-linking could be prepared. The plasma polymers containing anhydride groups that hydrolyzed fastly at air into carboxyl groups were deposited in kHz-frequency dielectric barrier discharge at atmospheric pressure from the mixture of maleic anhydride and acetylene. The variation of the flow rate ratio was used to optimize the stability of films together with the amount of functional groups. Amine and carboxyl plasma polymers proved to be useful for the preparation of immunosensors based either on the principle of quartz crystal microbalance or surface plasmon resonance because in both these methods it is necessary to prepare a stable and reactive film on the gold surface. The amine films were also tested for the cultivation of human dermal fibroblasts and mouse myoblasts. It was identified that the water stability of the films is very important for succesfull experiments