| AVS 63rd International Symposium & Exhibition | |
| Nanometer-scale Science and Technology | Thursday Sessions |
| Session NS+BI-ThA |
| Session: | Applied Nanoscale Microscopy Techniques/Biomaterial Interfaces – New Advances |
| Presenter: | Dmitri Petrovykh, International Iberian Nanotechnology Laboratory, Portugal |
| Authors: | V. Mugnaini, International Iberian Nanotechnology Laboratory, Portugal D.Y. Petrovykh, International Iberian Nanotechnology Laboratory, Portugal |
| Correspondent: | Click to Email |
We systematically investigated Quartz Crystal Microbalance with Dissipation (QCM-D) measurements in aqueous solutions of model strong electrolytes that are commonly used in experiments with biological surfaces. In particular, we examined the quantitative behavior of both frequency and dissipation responses in transitions between two different aqueous solutions.
The abrupt changes in the QCM-D responses upon such transitions are sometimes referred to as “jumps” associated with switching the bulk properties of the fluid flowing through the QCM-D cell. Switching between fluids of different compositions may be important in a variety of QCM-D measurements for biointerfaces, e.g., when switching between a baseline/rinsing solution and a measurement solution, or switching between optimal buffers used for probe immobilization and for biorecognition steps [1-3]. In specialized quantitative biointerface measurements, such as measuring stabilities of DNA hybrids [2-3], switching among different solutions multiple times actually provides the basis for the measurement.
In typical QCM-D measurements, the baseline is reset after a “bulk jump”, so the data are typically only quantified between any transients, but not across them, i.e., quantification is carried out for a constant fluid composition, but not between different fluids. By considering the underlying viscoelastic formalism [4-5], we demonstrate in a series of systematic measurements for solutions of strong electrolytes that the QCM-D responses upon switching between different solutions can be quantitatively predicted and exhibit interesting scaling behavior. Classical theory of the viscosity of electrolyte solutions provides additional insight into correlations between the results measured for different salts.
[1] D. Y. Petrovykh, H. Kimura-Suda, L. J. Whitman, M. J. Tarlov, J. Am. Chem. Soc. 125, 5219 (2003)
[2] S. M. Schreiner, D. F. Shudy, A. L. Hatch, A. Opdahl, L. J. Whitman, D. Y. Petrovykh, Anal. Chem. 82, 2803 (2010)
[3] S. M. Schreiner, A. L. Hatch, D. F. Shudy, D. R. Howard, C. Howell, J. Zhao, P. Koelsch, M. Zharnikov, D. Y. Petrovykh, A. Opdahl, Anal. Chem. 83, 4288 (2011)
[4] K. K. Kanazawa, J. G. Gordon II, Anal. Chim. Acta, 175, 99 (1985)
[5] M. Rodahl, F. Höök, A. Krozer, P. Brzezinski, B. Kasemo, Rev. Sci. Instrum. 66, 3924 (1995)