| AVS 63rd International Symposium & Exhibition | |
| Biomaterial Interfaces | Monday Sessions |
| Session BI+AS-MoM |
| Session: | Biomolecules and Cells at Interfaces |
| Presenter: | Axel Rosenhahn, Ruhr-University Bochum, Germany |
| Authors: | K. Nolte, Ruhr-University Bochum, Germany M. Alles, University of Heidelberg, Germany M.P. Arpa-Sancet, University of Heidelberg, Germany C. Christophis, University of Heidelberg, Germany A. Rosenhahn, Ruhr-University Bochum, Germany |
| Correspondent: | Click to Email |
When new Materials are developed to control and influence Biofilm growth, the ability of biofilm formers to firmly adhere to the coatings is one key property. Several techniques have been developed in the past to probe attachment strength of cells [1]. Especially microfluidic test systems [2] offer several advantages, such as small sample area, small amounts of target species, and high throughput. We developed microfluidic assays that allow to test bacterial and diatom adhesion on coatings [3,4]. Cells are driven through a microchannel at a precisely controlled flow rate and at a constant concentration and both, accumulation and detachment can be monitored by video microscopy. Using self-assembled monolayers as model surfaces we were able to show that the adhesion strength correlates with the accumulation dynamics if an appropriate shear stress is applied. Based on this finding, a parallelized microfluidic system has been developed that allows simultaneous, comparative testing of materials. Due to the modular assembly of the setup, not only model surfaces and thin organic films, but also practical coatings can be analyzed.
[1] L. Marcotte, M. Tabrizian, ITBM-RBM 2008, 29, 77
[2] D.P. Bakker, A. van der Plaats, G.J. Verkerke, H.J. Busscher, H.C. van der Mei, Appl. Envir. Microbiol. 2003, 69(10), 6280
[3] M. Arpa-Sancet, C. Christophis, A. Rosenhahn, Biointerphases 2012, 7, 2
[4] M. Alles, A. Rosenhahn, Biofouling. 2015, 31, 469–480.