| AVS 63rd International Symposium & Exhibition | |
| Biomaterial Interfaces | Tuesday Sessions |
| Session BI+AS+SA-TuA |
| Session: | Biophysics and Characterization of Biological and Biomaterial Surfaces |
| Presenter: | Giacomo Ceccone, EC-JRC-IHCP, Italy |
| Authors: | I. Ojea, EC-JRC-IHCP, Italy R. Capomaccio, EC-JRC-IHCP, Italy L. Calzolai, EC-JRC-IHCP, Italy D. Gilliland, EC-JRC-IHCP, Italy P. Colpo, EC-JRC-IHCP, Italy G. Ceccone, EC-JRC-IHCP, Italy G. Siligardi, Diamond Light Source, Oxfordshire, UK R. Hussein, Diamond Light Source, Oxfordshire, UK |
| Correspondent: | Click to Email |
The characterisation of protein corona formed around nanoparticles is a very important and challenging issue in the investigation of nanomaterials behaviour in biological environment and has been studied by many authors [1, 2, 3,4].
On the other hand, it is recognized that detailed physico-chemical characterization of nanomaterials is becoming increasingly important both from the technological and from health and safety point of view. Moreover, an incomplete characterisation may inhibit or delay the scientific and technological impact of nanoscience and nanotechnology [5]. In this respect, surface chemical analysis methods, such as X-ray Photoelectron Spectroscopy (XPS) and Time of Flight Secondary Ion Mass Spectrometry, can provide an important contribution to more fully characterizing nanomaterials [6].
In this work, we have investigated the interaction of human serum albumin (HSA) with gold nanoparticles (AuNPs) functionalized with thiols. In particular, 15 nm AuNPs functionalized with PEG thiols have been studied before and after interaction with HSA.
The different steps of sample preparation have been characterised by DLS, CPS and TEM, whilst the surface chemistry has been mainly assessed by XPS. Finally, the interaction between nanoparticles and HSA has been studied by Synchrotron Radiation Circular Dichroism (SRCD) to gather information on the protein structure [7]. In particular, XPS and ToF-SIMS data revealed the presence of HSA on pegylated nanoparticles, whilst the use of SRCD in combination with separation techniques allowed the determination of the structure and morphology of HSA-AuNPs complexes [8]. Moreover, SRCD experiments indicate that AuNPs increase the UV and thermal stability of HSA.
[1] Bigdeli A., et al., ACSNano, 2016, DOI: 10.1021/acsnano.6b00261
[2] Huang R., et al. Nanoscale, 2013, 5, 6928–6935
[3] Winzen S., et al., Nanoscale, 2015, 7, 2992–3001
[4] Lynch I. and Dawson K.A., NanoToday, 2008, 3(1-2) 42-47
[5] Baer D, et al., Anal. Bioanal. Chem., 2010, 396(3), 983–1002
[6] Grainger D and Castner D, Adv. Mater., 2008, 20, 867–877
[7] Laera S., et al., Nanoletters, 2011, 11, 4480–4484.
[8] Capomaccio R., et al., Nanoscale, 2015, 7, 17653–17657.