| AVS 63rd International Symposium & Exhibition | |
| Biomaterial Interfaces | Thursday Sessions |
| Session BI+AS+SA-ThM |
| Session: | Synthesis and Processing of Biomaterials/Biologically Inspired Materials |
| Presenter: | Jean Paul Allain, University of Illinois at Urbana-Champaign |
| Authors: | J.P. Allain, University of Illinois at Urbana-Champaign A.R. Shetty, University of Illinois at Urbana-Champaign S. Arias, University of Illinois at Urbana Champaign A. Barnwell, University of Illinois at Urbana Champaign F. Echeverria, University of Antioquia, Colombia L.F. Berrio, University of Antioquia, Colombia |
| Correspondent: | Click to Email |
An important aspect of tissue engineering is to create a favorable extracellular microenvironment, mainly the extracellular matrix (ECM) which can guide cell differentiation and tissue regeneration. The ECM consists of a number of cues that can be guided by surface topography and matrix stiffness [1]. Recent studies [2,3] have demonstrated that depending on the type of surface structuring and patterning, cell adhesion can be controlled with potential applications in smart cell culture systems and biosensors. Many of the desired biomaterial properties that require a combination of metal alloy and soft material interfaces cannot be processed with conventional bottom-up techniques. Directed irradiation synthesis (DIS) address this limitation by introducing a synthesis process that is scalable to high-volume manufacturing by virtue of its intrinsic large-area simultaneous exposure of materials surfaces and interfaces.
In this study, we have employed directed irradiation synthesis to induce nanostructure formation on two commonly used biomaterials: 1) Ti6Al4V and 2) magnesium (Mg). The goal is to examine the role of surface nanostructuring on the stimulation of cells and tissues in order to provide important cues for tissue regeneration as well as guarantee a good corrosion resistance and to minimize bacteria adhesion. Detailed characterization, establishing processing conditions and correlating them to surface and biomaterial properties have been successfully performed on nanostructured medical grade Ti6Al4V and Mg. These irradiated surfaces were biologically evaluated by using human aortic smooth muscle cells (HASMCs) for cytotoxicity and cell/surface adhesion and interactions. This analysis allowed us to determine connections with processing, structure, surface energy, and biointerface properties. Biological response of these new surfaces has also lead us, for the first time, to establish correlations between nanostructuring by DIS and cell stimulation, as well as to show the real potential of these new surfaces to favorably stimulate cells and tissues different than bone. The corrosion behavior of these biomaterials in a phosphate buffered simulated body fluid (SBF) has also been investigated for bone implant application.
References:
1) Evelyn, K.F., Darling, E.M., Kulangara, K., Guilak, F., and Leong K.W., Biomaterials2010,
31, 1299–1306.
2) Guasch, J. Diemer, J., Riahinezhad, H., Neubauer, S., Kessler, H., and Spatz, J.P., Chem.
Mater. 2016, 28, 1806–1815
3) Slater, J.H, Boyce, P.J, Jancaitis, M.P., Gaubert, H.E, Chang, A.L., Markey, M.K., and Frey
W., ACS Appl. Mater. Interfaces 2015, 7, 4390–4400.