| AVS 62nd International Symposium & Exhibition | |
| Surface Modification of Materials by Plasmas for Medical Purposes Focus Topic | Thursday Sessions |
| Session SM+AS+BI+PS-ThM |
| Session: | Plasma Processing of Biomaterials |
| Presenter: | Masaaki Nagatsu, Shizuoka University, Japan |
| Authors: | M. Nagatsu, Shizuoka University, Japan H. Chou, Shizuoka University, Japan A. Viswan, Shizuoka University, Japan T. Abuzairi, Shizuoka University, Japan M. Okada, Shizuoka University, Japan M.A. Ciolan, Shizuoka University, Japan N.R. Poespawati, University of Indonesia R.W. Purnamaningsih, University of Indonesia A. Sakudo, University of the Ryukyus, Japan S. Bhattacharjee, Indian Institute of Technology, Kanpur, India |
| Correspondent: | Click to Email |
In this study, we will present the recent experimental results on plasma surface functionalization of nano-structured materials for bio-medical applications.
First, with the graphite-encapsulated magnetic nanoparticles(MNPs), we studied the surface functionalization by using the Ar plasma pre-treatment followed by NH3 plasma post-treatment, to introduce the amino groups onto the surface of the nanoparticles.1) The amino group population of each nanoparticle with a typical diameter of 20 nm was evaluated by using the conventional chemical technique using SPDP and DTT solutions and we obtained about 8 x 104 amino groups per nanoparticle.2) Immobilization of the antibody of influenza virus onto the surface of amino-modulated magnetic nanoparticles was then performed for aiming at studying the feasibility of collection and condensation of virus. After magnetic separation, we succeeded in a significant concentration of the influenza virus number compared with that of the initial sample.3) Using the same method, we also demonstrated a higher concentration of Salmonella about 70 times higher than that of initial sample by the magnetic separation.4) The present results suggest the feasibility of the proposed plasma surface functionalized MNPs for rapid concentration of influenza virus or various bacteria.
As the second topic, the selective ultrafine surface modification of functional groups onto the polymeric substrate or vertically aligned CNT dot-array with a dot size of several μm was investigated using the atmospheric pressure plasma jet with a nano/micro-sized capillary. The micro-sized surface modification of amino or carboxyl groups introduced onto the CNT dot-array were confirmed by the fluorescence labelling technique.5) With fluorescence-labeled avidin molecules, we also confirmed efficient capturing of avidin molecules by the biotin-immobilized CNT dot array through strong biotin-avidin binding process. The present result supports the feasibility of future biochip sensor to detect specific protein, virus or bacteria. In addition to these results, the other experimental results will be presented and discussed at the conference.
References
1) T. E. Saraswati, A. Ogino, M. Nagatsu, Carbon, 50 (2012) pp.1253-1261.
2) T. E. Saraswati, S. Tsumura, and M. Nagatsu, Jpn. J. Appl. Phys. 53 (2014) 010205(5 pages).
3) A. Sakudo, H. Chou, K. Ikuta, and M. Nagatsu, Bioorg. Med. Chem. Lett. 25 (2015) pp.1876–1879.
4) A. Sakudo, H. Chou, and M. Nagatsu, Bioorg. Med. Chem. Lett., 25 (2015) pp. 1012-1016
5) T. Abuzairi, M. Okada, Y. Mochizuki, N. R. Poespawati, R. Wigajatri and M. Nagatsu, Carbon, 89 (2015) pp. 208-216.