| AVS 62nd International Symposium & Exhibition | |
| In-Situ Spectroscopy and Microscopy Focus Topic | Wednesday Sessions |
| Session IS+SS+NS+BI+VT+MN+AS-WeA |
| Session: | In situ Imaging of Liquids using Microfluidics |
| Presenter: | James Evans, Pacific Northwest National Laboratory |
| Authors: | J.E. Evans, Pacific Northwest National Laboratory C. Smallwood, Pacific Northwest National Laboratory |
| Correspondent: | Click to Email |
Biological organisms have evolved a number of spatially localized and highly orchestrated mechanisms for interacting with their environment. Since no single instrument is capable of probing the entire multidimensional landscape, it is not surprising that one of the grand challenges in biology remains the determination of how dynamics across these scales lead to observed phenotypes.
Therefore, there is a need for in-situ correlative multimodal and multiscale imaging to fully understand biological phenomena and how chemical or structural changes at the molecular level impact the whole organism. We have been advancing new methods for both cryogenic and in-situ correlative analysis of biological samples using electron, ion, optical and x-ray modalities. Central to this work is the development of new micro- and nanofluidic chambers that enable in-situ observations within precisely controlled liquid-flow environments. In this talk I will review the design of these new chambers, highlight current science applications and outline our future goals for adding additional functionality and expanding the versatility of the devices to other disciplines.