| AVS 62nd International Symposium & Exhibition | |
| Spectroscopic Ellipsometry Focus Topic | Thursday Sessions |
| Session EL+EM+EN-ThM |
| Session: | Spectroscopic Ellipsometry: Novel Applications and Theoretical Approaches |
| Presenter: | Gang Jin, Institute of Mechanics, Chinese Academy of Sciences, China |
| Authors: | Y. Niu, Institute of Mechanics, Chinese Academy of Sciences, China G. Jin, Institute of Mechanics, Chinese Academy of Sciences, China |
| Correspondent: | Click to Email |
The concept of biosensor based on imaging ellipsometry (BIE) was proposed in 1995 [1, 2]. With the development in recent 20 years, it has been formed an automatic analysis technique for detecting biomolecule detection interaction with merits of rapid, label-free, quantitative, high throughput and real-time. Its principle, methodology, biosensor system and biomedical applications are reviewed in this report.
A BIE system can be divided into four parts: the microfluidic array reactor, the imaging ellipsometer, the control system, and the biosensor database. The microfluidic array reactor serves to fabricate the protein microarray and accommodate biomolecular interactions. Using the microfluidic array reactor, various ligands are immobilized to different cells to form a sensing array, and each sensing surface can be prepared homogeneously under the flow condition. The imaging ellipsometer acts as a reader for data acquisition from the microarray. Since imaging ellipsometry is sensitive to slight variations of optical thickness, it can be used to visualize ultra-thin films and the change of molecular mass surface concentration. The control system combines the reactor with the imaging ellipsometer and functions to control the hardware’s mechanical motion and obtain results in images, while the biosensor database is to aid BIE users in determining optimized experimental conditions and comparing previous test data.
The sensitivity and flexibility of the biosensor is very important for practical purpose, especially in biomedical fields. The sensitivity depends not only upon the resolution of imaging ellipsometry but also upon the bio-system of ligand-receptor on the microarray that is the bioactivity and its act related to the ligand screen, ligand immobilization, unspecific blocking and interaction conditions, etc. The flexibility mainly depends on the mechanical, electrical, informatics and biological control. So far, a serviceable engineering system of the biosensor and some bio-systems is installed available for more applications, especially for high throughput protein analysis, such as antibody screening [3], disease markers serological detection [4] and joint detection of tumor markers [5] as well as virus infection identification [6-7].
References
[1] G. Jin, et al., Anal. Biochem. 232, 69 (1995).
[2] G. Jin, R. Jansson, and H. Arwin, Rev. Sci. Instrum.67, 2930 (1996).
[3] Y. Niu, et al., Thin Solid Films519 2768 (2011).
[4] C. Qi, et al., J. Viral Hepatitis16, 822 (2009).
[5] Y. Niu , et al., Thin Solid Films571, 453 (2014).
[6] C. Qi, et al., Biosensors & Bioelectronics25, 1530 (2010).
[7] C. Qi, et al., Virus Res.140, 79 (2009).