| AVS 62nd International Symposium & Exhibition | |
| Biomaterial Interfaces | Wednesday Sessions |
| Session BI-WeM |
| Session: | Biomolecules at Interfaces |
| Presenter: | Anouk Galtayries, Chimie ParisTech, France |
| Authors: | A. Galtayries, Chimie ParisTech, France V. Semetey, Chimie ParisTech, France A. Dellinger, Chimie ParisTech, France |
| Correspondent: | Click to Email |
The aim of this work is to design surfaces allowing controlling biomolecule adhesion by the study of protein adsorption and cell adhesion. In order to answer this challenge,: the optimization of grafting conditions using the thiol-ene reaction of thiol-terminated ethylene glycol (EG) chains (Oligo-EG or Poly-EG) on a undecenyltrichlorosilane self-assembled monolayer was investigated [1], with the help of surface characterization (angle contact measurement, ellipsometry, fluorescence microscopy, attenuated total reflection infrared IR-ATR, X-ray Photoelectron Spectroscopy XPS, Time-of-Flight Secondary Ion Mass Spectrometry ToF-SIMS) after each reaction step.
Varying different reaction parameters in the methodological investigation of thiol-ene grafting conditions exhibits the development of a bilayer structured system after a 1 minute reaction time as regards OEG grafting, and 1 hour time for PEG grafting. By using different passivated substrates (model silicon single crystal, polycrystalline titanium), different OEG-thiol or PEG-thiol molecules (from 7 to 220 ethylene unit long, methyl-, carboxyl- or amine-terminated), we highlight the range of available versions of this strategy. The terminal chemical functions lead on demand either to protein adsorption inhibition or to biomolecule adsorption, bovine serum albumin (BSA) or fibronectin (Fn) giving access to specific adhesion.
By controlling the light-exposed areas (100 mm-large bands or half-moon surfaces), the photochemistry occurring during the thiol-ene grafting allows to design surface patterning for addressing both protein adsorption and cell adhesion on such sample biointerface on metal oxides.
[1] ``A Facile and Versatile Approach to Design Self-Assembled Monolayers on Glass using Thiol-ene Chemistry'', B. Oberleitner, A. Dellinger, M. Déforet, A. Galtayries, A.-S. Castanet,V. Semetey, Chemical Communication, 49, 1615-1617 (2013).