| AVS 62nd International Symposium & Exhibition | |
| Biomaterial Interfaces | Wednesday Sessions |
| Session BI-WeA |
| Session: | Biophysics, Membranes and Nanoscale Biological Interfaces |
| Presenter: | Noo Li Jeon, Seoul National University, Korea |
| Correspondent: | Click to Email |
Vasculature is a fundamental building block of tissues. In order to design and fabricate in-vitro organ-on-chip systems, vasculature needs to be integrated with the micro-tissue constructs.
This presentation will describe the development of a novel microfluidic device with perfusable network of blood vessels that: 1) reproduce angiogenesis and vasculogenesis, 2) allow formation of network of perfusable endothelial vessels in 3D tissue, 3) allow observation of cancer and immune cell intravasation and extravasation, and 3) allow measurement of microvessel permeability. Cancer cells can be introduced and their intravasation and extravasation can be observed with live-cell imaging for extended periods and thus control the tumor microenvironment for investing different steps of metastatic cascade. Characterization of the vessels with immuno-histochemical techniques show that tight-junctions form between the endothelial cells and laminin and collagen IV are deposited along the length of the vessel.
The development of vascularized micro-tissue represents a step forward in building organ-on-chip systems. Drug screening and other assays can be performed using live-cell imaging and immunohistochemistry techniques enabling high throughput testing of multiple conditions simultaneously.