| AVS 62nd International Symposium & Exhibition | |
| Biomaterial Interfaces | Tuesday Sessions |
| Session BI-TuA |
| Session: | Cells and Microorganisms at Surfaces |
| Presenter: | Adoracion Pegalajar-Jurado, Colorado State University |
| Authors: | A. Pegalajar-Jurado, Colorado State University M.J. Hawker, Colorado State University M.N. Mann, Colorado State University E.R. Fisher, Colorado State University |
| Correspondent: | Click to Email |
Biofouling causes severe and costly problems in industries including, but not limited to water filtration, food packaging and preservation, marine operations and biomedical devices. Depending on the industrial context, the term biofouling assumes different meanings including bacterial attachment and biofilm formation, undesired protein adsorption, or prevention of cell growth and tissue regeneration. Nevertheless, the process commences with undesired interactions of biological agents with the material surface.Consequently, the ability to tune surface properties to tailor biological response highlights an exceptional route towards preventing issues associated with biofouling. Although surface micro- and nanotopography, surface free energy, and surface chemistry are known to affect biological agent-surface interactions, this presentation will focus specifically on the effects of surface chemical modifications of 3D constructs (i.e. drug delivery systems and polymeric membranes and scaffolds) on biological responses. Among others, plasma surface modification offers a tunable and versatile parameter space for tailored and reproducible surface modification while retaining the morphology of the material, to produce bio-nonreactive materials (limit bacterial and cell attachment and low cytotoxicity). On 2D substrates, plasma polymerized cineole films have demonstrated limited Escherichia coli (E. coli) attachment over 18 hours and non-cytotoxic to mammalian fibroblast .1 Herein, such films were used to conformally encapsulate 3D constructs. Results from both E. coli attachment studies as well as cytotoxicity studies will be presented. Alternatively, allylamine/allyl alcohol plasma copolymerized films applied to 3D materials and water plasma treated nitric-oxide releasing materials will be included as bio-reactive materials. In this case, human dermal fibroblast attachment and growth was enhanced in comparison to unmodified materials. Through these model systems, we will explore the use of plasma surface modification to minimise fouling and/or enhance biocompatibility of a 3D material resulting in the extension of device lifetime, and enhancement of performance.
Keywords: plasma surface modification, bio-reactive, bio-nonreactive
1 Pegalajar-Jurado, A., Easton, C. D., Styan, K. E., McArthur, S. L., Journal of Materials Chemistry B, 2, no. 31 (2014): 4993-5002.