AVS 61st International Symposium & Exhibition
    Biomaterial Interfaces Tuesday Sessions
       Session BI+AS-TuA

Invited Paper BI+AS-TuA3
Simultaneous 3D Detection of Organics for Intact Samples with Infrared Spectromicrotomography

Tuesday, November 11, 2014, 3:00 pm, Room 317

Session: Characterization of Biointerfaces
Presenter: Carol Hirschmugl, University of Wisconsin Milwaukee
Correspondent: Click to Email
The holy grail of chemical imaging is to provide spatially and temporally resolved information about heterogeneous samples on relevant scales. Synchrotron-based Fourier Transform infrared imaging1 combines rapid, non-destructive chemical detection with morphology at the micrometer scale, to provide value added results to standard analytical methods. Hyperspectral cubes of (x,y, z, Abs ()) are obtained employing spectromicrotomography2, a label free approach, it inherently evaluates a broad array of wide organic materials, with minimal sample preparation and modification. Examples presented here (polymer composites, single cells and colonies of cells) demonstrate the broad applicability of this approach to detect complex chemical information of intact samples.

References

1 Nasse, M. J., Walsh, M. J., Mattson, E. C., Reininger, R., Kajdacsy-Balla, A., Macias, V., Bhargava, R., and Hirschmugl, C. J. (2011) Nat.Methods 8, 413-416

2 Martin, M. C., Dabat-Blondeau, C., Unger, M., Sedlmair, J., Parkinson, D. Y., Bechtel, H. A., Illman, B., Castro, J. M., Keiluweit, M., Buschke, D., Ogle, B., Nasse, M. J., and Hirschmugl, C. J. (2013) Nat.Methods 10, 861-864

Acknowledgements

This work was supported by the US NSF under awards CHE-0832298, CHE-1112433, and DMR-0619759, the Research Growth Initiative of the University of Wisconsin–Milwaukee, and is based on research conducted at the Synchrotron Radiation Center, University of Wisconsin–Madison which is supported by the University of Wisconsin– Milwaukee and University of Wisconsin–Madison.